Earticle

초록

영어

The present study investigated whether treadmill exercise with bone marrow stromal cells (BMSCs) transplantation increase expression lev-el of protein synthesis-related molecules in the soleus muscle after spi-nal cord injury (SCI). The spinal cord contusion injury was performed at the T9–10 level using the impactor (10 g×25 mm). BMSCs were cultured from femur and tibia of 4-week-old rats and then transplanted directly into the lesion 1-week post injury. The rats in exercise group were walk-ing on treadmill device for 6 days per a week during 6 weeks. Prepared soleus muscles were used for examining mechanisms of protein syn-thesis after SCI. Myostatin induction level was increased by SCI, but BMSCs engrafting after SCI decreased compared to SCI group. Combi-nation of treadmill exercise with BMSCs showed more potent decre-ment on myostatin expression. Protein kinase B (Akt) and mammalian target of rapamycin (mTOR) levels were significantly increased in SCI and BMSCs transplantation group compared to SCI group. Combination of treadmill exercise with BMSCs further facilitated expression levels of Akt and mTOR. Insulin-like growth factor-I (IGF-I) and phosphorylated cyclic adenosine monophosphate response element-binding protein (p-CREB) induction levels were more increased in SCI and BMSC trans-plantation group compared to SCI group. Combination of treadmill exer-cise with BMSCs further increased expression levels of IGF-I and p-CREB, although statistical significance was not appeared. Combining treadmill exercise with BMSCs transplantation might accelerate protein synthesis and hypertrophy in the soleus muscle after SCI through acti-vation of IGF-I/mTOR signaling pathway.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Animals and treatments
Spinal cord contusion injury
BMSCs culture and transplantation
Treadmill exercise protocol
Western blot analysis
Data analysis
RESULTS
Changes of myostatin expression level
Changes of Akt and mTOR levels
Changes of IGF-I and p-CREB levels
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• You-Mi Kim [ Sports Science Research Institution, Korea National Sport University, Seoul, Korea ]
• Eun-Sang Ji [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Il-Gyu Ko [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Jun-Jang Jin [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Yeong-Hyun Cho [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Tae-Beom Seo [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613