Earticle

초록

영어

Maternal separation causes depression and anxiety. Exercise amelio-rates maternal separation-induced depression. In this study, we investi-gated the effect of treadmill exercise on anxiety-like behavior in relation with glycogen synthase kinase 3 beta (GSK3β)/β-catenin pathway using maternal separation rat pups. For this study, elevated plus maze test, immunohistochemistry for serotonin (5-hydroxytryptamine, 5-HT), tryp-tophan hydroxylase (TPH), and western blot for total GSK3β (t-GSK3β), phosphorylated GSK3β (p-GSK3β), total β-catenin (t-β-catenin), and phosphorylated β-catenin (p-β-catenin) were conducted. The rat pups in the exercise groups were scheduled to run on a treadmill for 30 min once a day for 10 days, starting on postnatal day 21. For the rat pups in the fluoxetine-treated group, fluoxetine was orally administrated once a day for 10 consecutive days, starting on postnatal day 21. Anxiety-like behavior was appeared in the rat pups by maternal separation. Mater-nal separation suppressed 5-HT and TPH expression in the dorsal ra-phe. Maternal separation suppressed phosphorylation of GSK3β and increased phosphorylation of β-catenin in the hippocampus. However, treadmill exercise and fluoxetine treatment alleviated anxiety and in-creased 5-HT and TPH expression in the dorsal raphe. Treadmill exer-cise and fluoxetine treatment also enhanced GSK3β phosphorylation and suppressed β-catenin phosphorylation in the hippocampus. In this study, alleviating effect of treadmill exercise on maternal separation-in-duced anxiety appeared through enhancing 5-HT expression and GSK3β phosphorylation, and then inhibiting β-catenin phosphorylation. These results showed that treadmill exercise relieves anxiety through GSK3β/β-catenin pathway. Treadmill exercise showed similar amelio-rating effect on anxiety-like behavior as fluoxetine.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Experimental animals
Treadmill exercise protocol and treatment of fluoxetine
Elevated plus maze test
Tissue preparation
Immunohistochemistry
Western blot analysis
Data analysis
RESULTS
Anxiety-like behavior
Expression of 5-HT and TPH in the dorsal raphe
Expression of t-GSK3β and p-GSK3β in the hippocampus
Expression of t-β-catenin and p-β-catenin in the hippocampus
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Lin Ru Wang [ Department of Sport & Health Care, College of Art & Culture, Sangmyung University, Seoul, Korea ]
• Sang-Hoon Kim [ Department of Sport & Health Care, College of Art & Culture, Sangmyung University, Seoul, Korea ]
• Seung-Soo Baek [ Department of Sport & Health Care, College of Art & Culture, Sangmyung University, Seoul, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613