Lignin degrading enzymes from Lentinula edodes have broad substrate specificities, and therefore can degrade a variety of recalcitrant compounds. In this study, the lignolytic biodegradation was investigated in five different L. edodes fungi (Chunbaegko, Sanjo 303ho, Poongnyunko, Baekhwahyang, and Soohyangko). The fungi were evaluated for their ability to decolorize Remazol Brilliant Blue R (RBBR) in malt extract broth medium. Sanjo 303ho, Poongnyunko, Baekhwahyang, and Soohyangko rapidly decolorized RBBR within 7 days. The activities of manganese peroxidase (MnP) and laccase were determined in the absence and presence of lignin. Poongnyunko displayed the highest ligninolytic activity on day 7 of incubation (2,809 U/ mg and 2,230 U/mg for MnP and laccase, respectively).
목차
ABSTRACT 서론 재료 및 방법 공시균주 RBBR을 이용한 균주 탈색능 측정 목질섬유소 분해효소의 활성 검정 Filter paper를 이용한 목질섬유소 분해능 측정 통계분석 결과 및 고찰 RBBR 탈색능과 lignin 분해능 비교 MnP와 laccase의 목질섬유소 분해활성 검정 목질섬유소 분해효소의 Filter paper 분해활성 비교 요약 REFERENCES
키워드
Lentinula edodesLignocelluloseRemazol Brilliant Blue R (RBBR)LaccaseManganese peroxidase (MnP)
저자
정상욱 [ Sang-Wook Jeong | 장흥군버섯산업연구원 ]
장은경 [ Eun-Gyeong Jang | 장흥군버섯산업연구원 ]
정찬문 [ Chan–Mun Jeong | 장흥군버섯산업연구원 ]
고한규 [ Han-Gyu Ko | 산림버섯연구센터 ]
권혁우 [ Hyuk-Woo Kwon | 산림버섯연구센터 ]
반승언 [ Seung-Eon Ban | 장흥군버섯산업연구원 ]
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