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Preparation of cordycepin from Cordyceps militaris fruiting body : Optimization of extraction conditions and chromatographic separation

첫 페이지 보기
  • 발행기관
    한국버섯학회 바로가기
  • 간행물
    버섯 바로가기
  • 통권
    제22권 2호 통권 38호 (2018.10)바로가기
  • 페이지
    pp.68-68
  • 저자
    Ayman Turk, Sung Min Ko, Seon Beom Kim, Beom Seok Kim, Bang Yeon Hwang, Mi Kyeong Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A346978

원문정보

초록

영어
Cordycepin is one of the most crucial bioactive compounds produced by Cordyceps militaris and has exhibited antitumor activity in various cancers. In the present work, the response surface methodology (RSM) was used to determine optimum conditions for the extraction of cordycepin from C. militaris fruiting body. Extraction conditions, such as extraction solvent and extraction time were optimized using RSM. Analysis of variance (ANOVA) was applied and the significant effect of the factors and their interactions were tested at 95% confidence interval. Extraction solvent composition and time affected the extraction of cordycepin from C. militaris and the maximum amount of cordycepin was obtained when the samples were extracted using 50% methanol for 4 h. Furthermore, Diaion HP-20 column was successively used to isolate and purify cordycepin from C. militaris. Water crude extract of C. militaris was fractionated using Diaion HP-20 composed of water-methanol (W:M) and fraction (W:M 60:40) yielded 63.8% of cordycepin, and the fraction (W:M 80:20) produced 33.7 % consecutively, whereas the methanolic crude extract yielded 57 % and 38.6 % respectively. The purity of the prepared cordycepin was 93.2 % in one-step separation according to the high performance liquid chromatography (HPLC) analysis. The preparation method is simple, highly efficient, energy saving, and has been demonstrated to be effective for large preparations of cordycepin from crude extract with high purity and low equipment and operating costs.

키워드

Cordyceps militaris cordycepin Diaion HP-20 column high performance liquid chromatography (HPLC) response surface methodology (RSM).

저자

  • Ayman Turk [ College of Pharmacy, Chungbuk National University, Cheongju, 28160, Korea ]
  • Sung Min Ko [ C&G Agricultural Association, Sejong, 30067, Korea ]
  • Seon Beom Kim [ College of Pharmacy, Chungbuk National University, Cheongju, 28160, Korea ]
  • Beom Seok Kim [ C&G Agricultural Association, Sejong, 30067, Korea ]
  • Bang Yeon Hwang [ College of Pharmacy, Chungbuk National University, Cheongju, 28160, Korea ]
  • Mi Kyeong Lee [ College of Pharmacy, Chungbuk National University, Cheongju, 28160, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국버섯학회 [The Korean Society of Mushroom Science]
  • 설립연도
    1997
  • 분야
    농수해양>농학
  • 소개
    우리나라의 버섯 연구와 관련 산업의 발전에 기여하고 버섯관련 연구자와 관련산업 종사자간의 학술정보 등의 교류를 목적으로 한다.

간행물

  • 간행물명
    버섯 [Mushroom]
  • 간기
    반년간
  • 수록기간
    1997~2025
  • 십진분류
    KDC 525 DDC 635

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