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Development of molecular markers for discriminating the Lentinula edoes cultivars consumed in the Korean market

첫 페이지 보기
  • 발행기관
    한국버섯학회 바로가기
  • 간행물
    버섯 바로가기
  • 통권
    제22권 2호 통권 38호 (2018.10)바로가기
  • 페이지
    pp.33-33
  • 저자
    Jae-Gu Han, Jae-Han Cho, Mun Yhung Jung, Seung-Hyun Kim, Kang-Hyo Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A346930

원문정보

초록

영어
Lentinula edodes (Berk.) Pegler is a wood-decaying mushroom belonging to the family Marasmiaceae in Agaricales. Fresh and dried L. edodes has been widely used in the cuisines of Asian countries. The artificial production of L. edodes is usually conducted by using log cultivation and bag cultivation. A number of commercial cultivars optimized for each cultivating method have been developed. In the Korean market, Chinese and Japanese cultivars as well as domestic ones are circulated. Especially a huge amount of L. edodes media which are already inoculated and fully incubated are being imported from China throughout the year. In this study, the authors aimed to develop the specific molecular markers that are able to distinguish the L. edodes cultivars consumed in the domestic market in Korea. Next-generation sequencing data of all the L. edodes cultivars were generated using Illumina Hiseq. Sequencing reads were mapped onto the reference genomes B17 ver. 2 (PRJNA310971), and mitochondrial genome akiyama A567 (NC_018365) using a bioinformatic tool, BWA. SNP genotyping was performed by PICARD and GATK, and the gene annotation was conducted with the aid of SnpEff. To design the sensitive and specific molecular markers we filtered out the SNPs with depth <5, SNP ratio <0.9. As a result, we could find several combinations of nuclear and mitochondrial genetic markers discriminating L. edodes cultivars.

저자

  • Jae-Gu Han [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration ]
  • Jae-Han Cho [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration ]
  • Mun Yhung Jung [ Department of Crop Science, College of Sanghuh Life Science, Konkuk University ]
  • Seung-Hyun Kim [ Department of Food Science and Biotechnology, Graduate School, Woosuk University ]
  • Kang-Hyo Lee [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국버섯학회 [The Korean Society of Mushroom Science]
  • 설립연도
    1997
  • 분야
    농수해양>농학
  • 소개
    우리나라의 버섯 연구와 관련 산업의 발전에 기여하고 버섯관련 연구자와 관련산업 종사자간의 학술정보 등의 교류를 목적으로 한다.

간행물

  • 간행물명
    버섯 [Mushroom]
  • 간기
    반년간
  • 수록기간
    1997~2025
  • 십진분류
    KDC 525 DDC 635

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