Min-Keun Kim, Soon-Ae Sim, Ji-Hye Park, Su-Won Seuk, Jeong-Min Jeong, Dong-Seong Kim, Byeong-Jeong Lee, Kwang-Pyo Hong
언어
영어(ENG)
URL
https://www.earticle.net/Article/A346929
원문정보
초록
영어
Lentinulla edodes, knows as Oak mushroom, has been widely used of nutritional and medicinal purpose. This study was conducted to screen the suitable conditions for mycelial growth and to determine the phylogenetic relationship of 58 L. edodes strains. Optimal mycelial growth was observed at 25°C and minimum mycelial growth observed at 10°C and 30°C on PDA (Potato Dextrose Agar) media. Genetic diversity of L. edodes strains was assessed using ITS sequence analysis. A phylogenetic tree based on the ITS region sequences indicated that collected strains could be classified into three clusters. Sequence analysis of the ITS1-5.8S-ITS2 region of 57 strains of L. edodes demonstrated that the ITS region shares mostly 100.0% sequence homology. Interestingly, the ITS region of KNR3093 shares 96.0% sequence homology. The results showed that phylogenetic relationship among the collected strains of L. edodes is high, but genetic diversity that help to develope new variety is low.
저자
Min-Keun Kim [ Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Service ]
Soon-Ae Sim [ Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Service ]
Ji-Hye Park [ Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Service ]
Su-Won Seuk [ Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Service ]
Jeong-Min Jeong [ Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Service ]
Dong-Seong Kim [ Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Service ]
Byeong-Jeong Lee [ Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Service ]
Kwang-Pyo Hong [ Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Service ]