조직배양 기술을 이용한 괄루근(栝樓根, Trichosnathis Radix) 기원식물 하늘타리 부정근 배양에 대한 소고(小考)
A study on propagation of the Trichosnathis Radix (Trichosanthes kirilowii Maxim. Root) using tissue culture techniques
The genus Trichosanthes (Cucurbitaceae) is a perennial vine plant native to China, Japan, Mongolia and Taiwan. However, the species T. kirilowii Maxim. and T. kirilowii var. japonica Kitam. are native plants mostly in Korea and Japan. T. kirilowii, is used as a Korean and Chinese medicinal herb. However, the roots which are used in traditional medicine take several years to mature when cultivated in a traditional way. Fortunately, through tissue culture techniques, the roots can be produced in a shorter period of time. This in vitro study therefore was conducted to identify and establish the optimal medium and plant growth regulator type and concentrations suitable for adventitious root formation and biomass enhancement of T. kirilowii roots. The wild roots were collected, disinfected and cultured in MS and DJ solid and liquid agitator culture basal media supplemented with auxin-based hormone IBA to induce adventitious roots and increase the biomass within a minimal time frame. We expect to establish a system of root cultivation of T. kirilowii for the stable supply of substantial mass production in the future by providing a standard in vitro propagation protocol for the root cultivation.
목차
Abstract 서론 본론 1. 하늘타리의 기원 및 각국 약전비교 2. 하늘타리 기내배양방법의 종류 3. 하늘타리 기내배양 장단점 4. 하늘타리 한약자원식물의 기내배양 결론 감사의 글 참고문헌