Earticle

현재 위치 Home

Antioxidant Activity and Anti-inflammatory Effects of Raw and Processed Fruits and Vegetables

첫 페이지 보기
  • 발행기관
    국제인공지능학회(구 한국인터넷방송통신학회) 바로가기
  • 간행물
    The International Journal of Advanced Smart Convergence KCI 등재 바로가기
  • 통권
    Volume 7 Number 3 (2018.09)바로가기
  • 페이지
    pp.146-154
  • 저자
    Hyun-Kyoung Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A338367

※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

원문정보

초록

영어
In this study we investigated antioxidant and anti-inflammatory activities of Malus Domestica (apple), Pyrus Communis L. (pear), Daucus carota L. (carrot), Brassica oleracea var. (broccoli), Brassica oleracea var. capitata (cabbage) and Raphanus sativus L. (radish), that were obtained from local market. As these are common fruits and vegetables that are widely consumed, we aimed to investigate their beneficial properties especially the antioxidant and anti-inflammatory. The samples were processed by an indirect heating method and their properties were compared to their raw forms. Based on DPPH and ABTS assay, processed samples showed better antioxidant activity compared to raw samples, and processed pear sample had the best antioxidant activity. The anti-inflammatory activities of the samples were also investigated in LPS-treated RAW 264.7 cells. The mRNA expressions of pro-inflammatory mediators and cytokines (iNOS, COX-2, TNF-α, IL-1β and IL-6) were assessed by RT-PCR. Processed samples exhibited better inhibition of iNOS, compared to the raw forms. Processed broccoli and cabbage samples exhibited outstanding anti-inflammatory effects. The samples did not exhibit cytotoxicity against RAW 264.7 cells up to 1mg/ mL as shown in the cell viability assay. Taken together, processed broccoli and cabbage samples exhibited the strongest anti-inflammatory properties.

목차

Abstract
 1. Introduction
 2. Experiment Materials
  2.1 Reagents
  2.2 Sample Preparation
 3. Experiments Method
  3.1. DPPH Radical Scavenging Activity
  3.2 ABTS radical scavenging activity
  3.3 Cell culture
  3.4 Nitric oxide & MTT cell viability assay
  3.5 Reverse transcriptase polymerase chain reaction (RT-PCR)
  3.6 Statistical analysis
 4. Result and Discussion
  4.1. Processed samples exhibited stronger antioxidant activity than raw samples
  4.2 Variable anti-inflammatory activity of raw and processed samples
  4.3 Pro-inflammatory mediator and cytokine expressions in RAW 264.7 cells using Reverse-transcriptase Polymerase Chain Reaction (RT-PCR)
 5. Conclusion
 References

키워드

Antioxidant Anti-inflammation RAW 264.7 cells Fruits Vegetables

저자

  • Hyun-Kyoung Kim [ Department of Food Science and Engineering, Seowon University, Cheongju, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    국제인공지능학회(구 한국인터넷방송통신학회) [The International Association for Artificial Intelligence]
  • 설립연도
    2000
  • 분야
    공학>전자/정보통신공학
  • 소개
    인터넷방송, 인터넷 TV , 방송 통신 네트워크 및 관련 분야에 대한 국내는 물론 국제적인 학술, 기술의 진흥발전에 공헌하고 지식 정보화 사회에 기여하고자 한다.

간행물

  • 간행물명
    The International Journal of Advanced Smart Convergence
  • 간기
    계간
  • pISSN
    2288-2847
  • eISSN
    2288-2855
  • 수록기간
    2012~2025
  • 십진분류
    KDC 326 DDC 380

이 권호 내 다른 논문 / The International Journal of Advanced Smart Convergence Volume 7 Number 3

    피인용수 : 0(자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      페이지 저장