Earticle

초록

영어

This study investigated the effect of simulated altitude training on the changes of small intestinal mucosa barrier, bacterial overgrowth and inflammatory response in the small intestine of rat. Male 8-week-old Sprague-Dawley rats were randomly divided into four groups: normal oxygen sedentary group (n=30), normal oxygen exercise group (n=30), low oxygen sedentary group (n=30) and low oxygen exercise group (n=30). Exercise training was on a treadmill for 1 hr per day on days 3, 6, and 9 in the hypoxia condition. Hematological profiles, hematolxylin and eosin staining, fluorescence in situ hybridization, reverse transcrip-tion-polymerase chain reaction and Western blot were used to analyze the effect of simulated altitude training on the amount of bacteria, and expression of mRNA and protein. Simulated exercise training signifi-cantly increased red blood cells and hematocrit. The small intestinal mucosa barrier was significantly injured by the simulated altitude exer-cise training. Comparatively more bacterial growth was evident in the small intestine by the simulated altitude exercise training. mRNA levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) and pro-tein expression of nuclear factor-kappa B (NF-κB) were significantly el-evated by simulated altitude exercise training. These results suggest that the simulated altitude exercise training may impair the small intes-tinal mucosa barrier via elevation of bacterial growth and inflammatory cytokines (IL-6, TNF-α) and the up-regulation of NF-κB in the rats.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Animals
  Exercise training protocol
  Tissue isolation
  Hematoxylin and eosin staining
  Florescence in situ hybridization
  RT-PCT
  Western blot
  Statistical analyses
 RESULTS
  Effect of simulated altitude training on body weight
  Effect of simulated altitude training on hematological profiles
  Effect of simulated altitude training on intestinal mucosa barrier
  Effect of simulated altitude training on bacteria in the small intestine
  Effect of simulated altitude training on mRNA levels of TNF-α and IL-6
  Effect of simulated altitude training on small intestineNF-κB
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Meng Li [ Department of Sport Science, Chungnam National University, Daejeon, Korea ]
• Tianyu Han [ Sports Science School, Beijing Sport University, Beijing, China ]
• Weijia Zhang [ Sports Science School, Beijing Sport University, Beijing, China ]
• Wei Li [ Department of Sport Science, Chungnam National University, Daejeon, Korea ]
• Yang Hu [ Sports Science School, Beijing Sport University, Beijing, China ]
• Sang Ki Lee [ Department of Sport Science, Chungnam National University, Daejeon, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613