Earticle

초록

영어

Traumatic brain injury (TBI) causes deficit in spatial learning and mem-ory function. Physical activity ameliorates neurological dysfunction af-ter TBI. We investigated the effect of late starting treadmill exercise on spatial learning ability in relation with cAMP-response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) signaling pathway using TBI rats. For this study, radial 8-arm maze test, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end label-ing) staining, caspase-3 immunohistochemistry, and western blot for Bax, Bcl-2, BDNF, tyrosine kinase B (TrkB), CREB, and phosphorylated CREP (p-CREB) were performed. TBI was induced by an electromag-netic-controlled cortical impact. The rats in the exercise groups were scheduled to run on a treadmill for 30 min once a day for 8 weeks start-ing 3 weeks after TBI. TBI impaired spatial learning ability and in-creased caspase-3 expression in the hippocampal dentate gyrus. TBI enhanced Bax expression and suppressed Bcl-2 expression in the hip-pocampus. TBI increased BDNF and TrkB expressions, resulted in the enhancement of p-CREB/CREB ratio in the hippocampus. However, treadmill exercise improved spatial learning ability, decreased caspase-3 expression, suppressed Bax expression, and increased Bcl-2 expression. Treadmill exercise alleviated TBI-induced over-expres-sion of BDNF and TrkB, which suppressed phosphorylation of CREB in the hippocampus. In the present study, late starting treadmill exercise improved spatial learning ability through suppressing TBI-induced acti-vation of CREB/BDNF/TrkB signaling pathway after TBI.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Animals and treatments
  Induction of TBI
  Exercise protocol
  Radial 8-arm maze test
  Tissue preparation
  Immunohistochemistry for caspase-3Immunohistochemistry was conducted to evaluate
  Western blotting
  Data analysis
 RESULTS
  Effect of treadmill exercise on the spatial learning ability in the radial 8-arm maze test
  Effect of treadmill exercise on caspase-3 expression in the hippocampal dentate gyrus
  Effect of treadmill exercise on Bax and Bcl-2 expressions in the hippocampus
  Effects of treadmill exercise on BDNF and TrkB expressions in the hippocampus
  Effects of treadmill exercise on the ratio of p-CREB to CREB in the hippocampus
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Il-Gyu Ko [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea, Kohwang Medical Research Institute, Kyung Hee University, Seoul, Korea ]
• Sung-Eun Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea, Kohwang Medical Research Institute, Kyung Hee University, Seoul, Korea ]
• Lakkyong Hwang [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea, Kohwang Medical Research Institute, Kyung Hee University, Seoul, Korea ]
• Jun-Jang Jin [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea, Kohwang Medical Research Institute, Kyung Hee University, Seoul, Korea ]
• Chang-Ju Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea, Kohwang Medical Research Institute, Kyung Hee University, Seoul, Korea ]
• Bo-Kyun Kim [ Department of Emergency Technology, College of Health Science, Gachon University, Incheon, Korea ]
• Hong Kim [ Department of Oriental Sports Medicine, College of Biomedical Science, Daegu Haany University, Gyeongsan, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613