P.oleracea, commonly known as a pigweed, is reported as a plant that lowers cholesterol increased by high-fat diet, is effective to improve the liver function (Won et al., 2011), activates antiobesity cell (Lee et al., 2006), and is effective to decrease the obesity cell. This research was aimed to observe antioxidative and antibacterial effect of P.oleracea on pathogenic bacteria and to study its antiobesity effect on mice. P.oleracea was extracted in two different ways-organic solvent and hot water-, and then liquefied culture and agar diffusion method were done on 6 types of bacteria-Bacillus cereus, Escherichia coli, Lactobacillus delbrueckii subsp. Bulgaricus, Staphylococcus aureus, Streptococcus thermophilus, Vibroparahaemolyticus- in order to check its antibacterial effect. Moreover, after P.oleracea extract, sugar solution, or the mixture of the two was provided to mice and their weight increase were checked, and through dissection afterward, their change in immunity was observed. Antioxidative ability was tested through the oxidation of iron and DPPH radical scavenging activity. As a result, P.oleracea showed antibacterial activity over 70% on 5 types of bacteria except E.coli, and mice that P.oleracea extract was provided with sugar had similar rate of weight increase to the control group. While weights of liver and kidney of mice in sugar provided group were heavier than the control group, P.oleracea extract provided group had similar weight to the control group, which shows that the extract help metabolism. Moreover, its antioxidative ability was confirmed by improving the oxidation of iron and DPPH radical scavenging activity. Conclusively, P.oleracea has high antibacterial activity on pathogenic bacteria, prevents from obesity caused by sugar, and has outstanding antioxidative effect.
목차
ABSTRACT 1. INTRODUCTION 2. THEORETICAL BACKGROUND 3. MATERIALS AND METHODS 3.1.Plant Collection and Extraction 3.2.Extraction of P.oleracea Using Organic Solvent (method I and II) 3.3.Extraction of P.oleracea Using Heat 3.4.Animal Preparation and Assignment of Treatments 3.5.Testing for Antibacterial Effect 3.6.Testing for Antioxidant Effect 3.7.Testing for Antiobesity effect 4. RESULTS 4.1.Antibacterial Effect of P.oleracea Extract 4.2.Antioxidant Effect 4.3.Antiobesity Effect 5. DISCUSSION 6. CONCLUSION 7. REFERENCE 8. APPENDIX
저자
Sang Hyun AHN [ Seoul science high school for gifted student, 7-1, Hyehwa-ro, Jongno-gu, Seoul, Korea ]
Corresponding author
Sang Hoon HAN [ Seoul science high school for gifted student, 7-1, Hyehwa-ro, Jongno-gu, Seoul, Korea ]
국제과학영재학회 [The Society for the International Gifted in Science]
설립연도
2006
분야
자연과학>자연과학일반
소개
과학영재를 위한 연구 활동을 통하여 과학영재를 발굴하고, 발굴된 과학영재가 과학 분야의 관련 연구 활동을 할 수 있도록 지원하여, 이들이 과학 관련 분야의 진로를 선택하도록 장려함으로써 국제경쟁력을 갖춘 우수 과학기술인을 배출하고 국가 경제발전 및 문화창달과 세계 과학 발전에 기여함을 목적으로 한다.
간행물
간행물명
APEC Youth Scientist Journal [APEC 청소년 과학학술지]
간기
반년간
pISSN
2005-5625
수록기간
2009~2022
십진분류
KDC 405DDC 505
이 권호 내 다른 논문 / APEC Youth Scientist Journal Vol. 6 No.2