EFFECT OF KOREAN TRADITIONAL ONGGI ON DETOXIFICATION OF CRYOPROTECTANT AND CRYOINJURY PREVENTION IN SPERM CRYOPRESERVATION
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- 발행기관
- 국제과학영재학회 바로가기
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- 간행물
- APEC Youth Scientist Journal 바로가기
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- 통권
- Vol. 6 No.2 (2014.07)바로가기
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- 페이지
- pp.56-73
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A328950
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원문정보
초록
- 영어
- Cell cryopreservation plays a central role in reproductive technology. A major impediment to the development of cell cryopreservation is that there has yet to be found cryopreservation solutions that successfully prevent destruction of cellular tissue caused by intracellular hydro expansion. This study utilized the Korean traditional bioceramic onggi in sperm cryopreservation. Onggi emits far-infrared ray that stimulates cellular biological activities, promotes antioxidant effects, and prevents cellular toxicity. This study explored onggi’s ability to maintain the structural integrity and motility of sperm when onggi exposure media are used as cryopreservation solutions. It also tested whether onggi exposure media could alleviate freeze damage caused by ice crystals and reduce toxicity of cryoprotectants. The results showed that onggi exposure media reduced sperm toxicity during freezing, minimized abrupt thermal changes during thawing, and promoted sperm motility before and after actual cryopreservation. Onggi exposure media could also alleviate the damaging toxicity effect that existing cryoprotectants inherently have on cells. In conclusion, if the conditions to produce a synergy effect between onggi and cryoprotectants during cryopreservation are met, the newfound properties of onggi may well aid in the advance of reproductive technology
목차
ABSTRACT
1. RESEARCH BACKGROUND & PURPOSE
2. PROCEDURE
2.1.Preparation of onggi exposure media
2.2.Freezing and thawing speed of onggi exposure media
2.3. Toxicity test of onggi exposure media by yeast fermentation
2.4. Extraction of mouse semen
2.5.Motility of mouse sperm in onggi exposure media
2.6. Motility of mouse sperm in cryoprotectant added onggi exposure media
2.7. Motility of post-frozen bovine sperm in onggi exposure media
3. DATE AND RESULTS
3.1. Freezing and thawing speed of onggi exposure media
3.2. Toxicity test of onggi exposure media by yeast fermentation
3.3. Motility of mouse sperm in onggi exposure media
3.4. Motility of mouse sperm in cryoprotectant added onggi exposure media
3.5. Motility of post-frozen bovine sperm in onggi exposure media
4. ANALYSIS
5. CONCLUSION
6. ACKNOWLEDGEMENTS
7. REFERENCES
저자
간행물 정보
발행기관
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- 발행기관명
- 국제과학영재학회 [The Society for the International Gifted in Science]
- 설립연도
- 2006
- 분야
- 자연과학>자연과학일반
- 소개
- 과학영재를 위한 연구 활동을 통하여 과학영재를 발굴하고, 발굴된 과학영재가 과학 분야의 관련 연구 활동을 할 수 있도록 지원하여, 이들이 과학 관련 분야의 진로를 선택하도록 장려함으로써 국제경쟁력을 갖춘 우수 과학기술인을 배출하고 국가 경제발전 및 문화창달과 세계 과학 발전에 기여함을 목적으로 한다.
간행물
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- 간행물명
- APEC Youth Scientist Journal [APEC 청소년 과학학술지]
- 간기
- 반년간
- pISSN
- 2005-5625
- 수록기간
- 2009~2022
- 십진분류
- KDC 405 DDC 505
이 권호 내 다른 논문 / APEC Youth Scientist Journal Vol. 6 No.2
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