As a result of culturing E.Coli in undernutrition condition, while there was no change in general growth, two types of E.Coli, energy storing E.Coli which contains resistance to different stressing environment and energy consuming E.Coli with increased energy consumption and decreased resistance to each stress, was able to be separated. Their character could be inherited even after repeated culture, and this was confirmed throughout the experiment; it was mutation in a perspective of epigenetics. Energy storing E.Coli obtained increased resistance to low temperature, thus if this E.Coli was used to store gene and transform character, the efficiency increased.
목차
ABSTRACT 1. Introduction 2. Materials and Methods Materials Method 3. Results and Discussion Preparation of Nutrient Agar Culture Medium and Nutrient Broth Culture Medium for Cultivation of E.Coli Re-Cultivation of E.Coli Kept in Refrigeration Dilution of NB Culture Medium to Specific Concentration Incubation of E.Coli on the NB Culture Medium Diluted to ½, ¼, and 1/8 of Original Concentration Reaction of E.Coli Samples, Cultivated in NB Culture Medium Diluted to ½, ¼, and 1/8 of its Original Concentration, to Antibiotics Reaction of E.Coli Samples, Cultivated in NB Culture Medium Diluted to ½, ¼, and 1/8 of its Original Concentration, to Hydrogen Bacteriophage Screening and Separation of Homogenous Bacteriophage Reaction of E.Coli Samples, Cultivated in NB Culture Medium Diluted to ½, ¼, and 1/8 of its Original Concentration, to Bacteriophage Infection Comparison of Energy Consumption in Energy Consuming type to Energy Storing Type of E. Coli Comparison of Toxin Production in Energy Consuming type and Energy Storing Type of E.Coli Transplant of Plasmid DNA, that Makes E.Coli Ampicillin-Tolerant, in Energy Saving E.Coli and Energy Consuming E.Coli Storage Period of Antibiotics-Tolerant plasmid DNA Transplanted Energy Storing E.Coli and Energy Consuming E.Coli 4. Conclusion 5. Reference
저자
Yoon Seo Koo [ Banpo High School, 71, Gomurae-ro, Seochogu, Seoul, Korea ]
Corresponding author
국제과학영재학회 [The Society for the International Gifted in Science]
설립연도
2006
분야
자연과학>자연과학일반
소개
과학영재를 위한 연구 활동을 통하여 과학영재를 발굴하고, 발굴된 과학영재가 과학 분야의 관련 연구 활동을 할 수 있도록 지원하여, 이들이 과학 관련 분야의 진로를 선택하도록 장려함으로써 국제경쟁력을 갖춘 우수 과학기술인을 배출하고 국가 경제발전 및 문화창달과 세계 과학 발전에 기여함을 목적으로 한다.
간행물
간행물명
APEC Youth Scientist Journal [APEC 청소년 과학학술지]
간기
반년간
pISSN
2005-5625
수록기간
2009~2022
십진분류
KDC 405DDC 505
이 권호 내 다른 논문 / APEC Youth Scientist Journal Vol. 8 No. 2