Earticle

초록

영어

Recommendations of therapeutic exercise in Duchenne muscular dystrophy are still controversial. The hypothesis that a low-intensity training (LIT) protocol leads to muscle adaptations on mdx mice model was tested. Dystrophic male mice with 8 weeks old were separated in exercised (mdxE, n= 8) and sedentary (mdxC, n= 8) groups. Wild-type mice were used as control (WT, n= 8) group. Exercised group underwent a LIT protocol (9 m/min, 30 min, 3 days/wk, 60 days) on a horizontal treadmill. At day 60 all animals were analyzed regarding parameters of markers of muscle lesion and extracellular matrix turnover of muscle tissue by collagens fibers on tibial anterior muscle. Histomorphometry attested that centrally located nuclei fibers and the coefficient of variance of minimal Feret’s diameter was similar in mdxE and mdxC groups (P= 1.000) and both groups presented higher mean values than WT group (P< 0.001). Fraction area of collagen fibers of mdxE group was lower than mdxC group (P= 0,027) and similar to WT group (P= 0,751). Intramuscular area of Col3 of the mdxE group was higher than mdxC and WT groups (P<0.001). Intramuscular area of Col1 on the mdxE group was similar to the mdxC group (P= 1.000) and both groups were higher than WT group (P< 0.001). LIT protocol had not influenced muscle injuries resulting from the dystrophin- deficiency membrane fragility. Although, LIT had provoked adaptations on extracellular matrix bringing higher elastic feature to dystrophic muscle tissue.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Experimental design
  LIT protocol
  Histological and histochemical analysis
  Immunohistochemistry analysis
  Qualitative sample analysis
  CLN quantification
  Minimal Feret’s diameter
  Intramuscular collagen fiber quantification
  Statistical analysis
 RESULTS
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Thaís P. Gaiad [ Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil ] Corresponding author
• Murilo X. Oliveira [ Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil ]
• Adalfredo R. Lobo-Jr. [ Institute of Agriculture Scinces, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Unaí, Brazil ]
• Lívia R. Libório [ Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil ]
• Priscilla A.F. Pinto [ Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil ]
• Danielle C. Fernandes [ Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil ]
• Ana Paula Santos [ Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil ]
• Carlos Eduardo Ambrósio [ Department of Veterinary Medicine, University of São Paulo (USP), Pirassununga, Brazil ]
• Alex Sander D. Machado [ Faculty of Medicine FAMED, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613