Earticle

초록

영어

Purpose: Embelin is a major active ingredient of Embelia ribes Burm, belonging to Myrsinaceae, and an important traditional medicinal plant of Indian origin. Embelin has been studied for anti-cancer, anti-inflammatory, and anti-oxidative effects. However, there have been no studies on the use of cosmetic raw materials for embelin. Therefore, in this study, we investigated the inhibition of apoptosis and the anti-inflmmatory effect to confim the possibility of embelin as a cosmetic material. Methods: Cell viability was measured by using water-soluble tetrazolium salt (WST- 1) assay. Quantitative real time polymerase chain reaction (qRT-PCR) was performed to quantitatively analyze the gene expression patterns of embelin-induced inhibition of apoptosis and anti-inflammatory effects in human dermal fibroblasts (HDFs). Apoptosis enzyme linked immunosorbent assay (ELISA) kit was used to confim the degree of apoptosis. 5,5', 6,6'-tetrachloro-1,1', 3,3'-tetraethylbenzimidazolylcarbo cyanine iodide (JC-1) mitochondrial membrane potential detection kit was used to measure mitochondrial membrane potential. Results: The WST-1 assay confirmed that the reduced cell viability by hydrogen peroxide (H2O2) was restored by embelin in a concentration-dependent manner. H2O2-induced apoptosis was restored by embelin, Bcl-2-associated X protein (BAX) gene expression was decreased and mitochondrial membrane potential difference was increased in a concentration dependent manner. It was confimed that embelin inhibits apoptosis in HDFs treated with H2O2. In order to investigate the anti-inflmmatory effect of embelin in H2O2-treated human dermal fibroblasts, gene expression of cyclooxygenase 2 (COX2), tumor necrosis factor α (TNFα), and interleukin 6 (IL6), which induce inflmmation, was examined and found to be decreased by embelin in a concentration dependent manner. Conclusion: These results suggest that embelin has a potential as an anti-aging cosmetic ingredient with inhibition of apoptosis and anti-inflmmatory properties.

한국어

목적: Embelin은 인도의 중요한 전통 약용 식물인 Embelia ribes Burm. (Myrsinaceae)의 주요 활성 성분으로 항암, 항염증 및 항산화 효과에 관련된 의약학 연구가 진행되었으나 화장품 원료 활용으로서의 연구는 전무한 실정이다. 그러므로 본 연구에서는embelin의 세포사멸 억제, 항염 효능를 검증하여 화장품 소재로서의 가능성을 확인하고자 하였다. 방법: 세포 생존율은 water soluble tetrazolium salt (WST-1) assay의 원리를 이용하여 측정하였다. Embelin에 의한 인간 진피섬유아세포 내에서 일어나는 세포사멸억제 효과와 항염 효과의 유전자 발현패턴을 정량적으로 분석하기 위하여 quantitative real time polymerase chain reaction (qRT-PCR)을 실행하였다. 세포사멸 정도를 확인하기 위해 Apoptosis ELISA kit를 사용하였고, 세포사멸 지표인 미토콘드리아막전위차를 측정하기 위해 5,5', 6,6'-tetrachloro-1,1', 3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) mitochondrial membrane potential detection kit를 사용하였다. 결과: WST-1 분석을 통해 과산화수소(H2O2)에 의한 감소된 세포 생존율이embelin에 의해 농도 의존적으로 회복되는 것을 확인하였다. H 2O2로 유도된 세포사멸이 embelin에 의해 회복되었고, 농도 의존적으로 세포 사멸을 촉진하는BAX 유전자 발현은 감소하였으며 미토콘드리아 막전위차는 증가하였다. 이를 통해 embelin이 H 2O2 처리된 인간 진피섬유아세포에서 세포사멸 억제 기능을 한다는 것을 확인하였다. H 2O2 처리된 인간 진피섬유아세포에서 embelin의항염증 효과를 알아보기 위해 염증을 유발하는 cyclooxygenase 2 (COX2), tumor necrosis factor α (TNFα), interleukin 6 (IL6)의유전자 발현을 분석한 결과, embelin에 의해 농도 의존적으로 유의하게 감소하는 것을 확인하였다. 결론: Embelin이 세포사멸 억제 효능 및 항염 효능을 가진 항노화 화장품 성분으로서 활용 가능성이 있다고 판단된다.

중국어

目的: Embelin是一种印度的重要传统药用植物Embelia ribes Burm. (Myrsinaceae)的主要活性成分，据医学研究报道具 有抗癌、抗炎症以及抗氧化作用，但作为化妆品原料的应用研究至今没有。因此通过调查embelin的细胞凋亡抑制以及 抗炎作用，探索作为化妆品原料的应用可行性。 方法: 利用water soluble tetrazolium salt (WST-1) assay原理测定细胞生 存率；为了定量分析人真皮成纤维细胞内细胞凋亡抑制效果和抗炎效果的遗传因子的表达，利用quantitative real time polymerase chain reaction (qRT-PCR)来测定。利用Apoptosis ELISA kit确认细胞凋亡程度，为测定细胞凋亡标志，即， 线粒体膜电位差利用5,5', 6,6'-tetrachloro-1,1', 3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) mitochondrial membrane potential detection kit来测定。 结果: 通过WST-1确认因H2O2减少的细胞生存率由embelin根据浓度依赖性逐 渐恢复；因H 2O2诱导细胞凋亡的细胞生存率由embelin根据浓度依赖性逐渐恢复，促进细胞凋亡的BAX遗传因子的表达 减少，线粒体膜电位差反而增加。通过以上研究确认embelin抑制H 2O2处理的人真皮成纤维细胞的细胞凋亡。在H2O2处 理的人真皮成纤维细胞中，确认embelin的抗炎症效果，分析炎症诱发因子cyclooxygenase 2 (COX2)、tumor necrosis factor (TNFα)、interleukin 6 (IL6)等遗传因子表达，其结果发现利用embelin处理后，以上遗传因子根据浓度依赖性减 少。 结论: Embelin抑制细胞凋亡，具有抗炎作用，因此作为抗衰老化妆品原料充分具有可行性。

목차

Abstract
 Introduction
 Methods
  1. 세포 배양
  2. 시료 처리
  3. Cell viability 측정
  4. RNA 추출과 cDNA 제조
  5. qRT-PCR
  6. 세포사멸 측정
  7. ΔΨm determination
  8. 통계처리
 Results and Discussion
  1. Embelin의 세포독성 및 세포보호 효과
  2. Embelin의 세포사멸 억제 효과
  3. Embelin의 항염 효과
 Conclusion
 Acknowledgements
 References
 국문초록
 참고문헌
 中文摘要

키워드

항산화 항염증 항노화 인간 진피섬유아세포 Embelin Embelin Anti-oxidative Anti-inflammatory Anti-aging Human dermal firoblasts Embelin 抗氧化 抗炎症 抗衰老 人真皮成纤维细胞

저자

• Hee-Jung Yong [ 용희정 | Department of Lecture, Beauty People Beauty School, Seongnam-si, Gyeonggi-do, Korea, Department of Bioengineering, Konkuk University, Seoul, Korea ]
• Gyu Ri Kim [ 김규리 | Department of Bioengineering, Konkuk University, Seoul, Korea ]
• Jin-Jung Ahn [ 안진정 | Department of Beauty Arts, Suwon Women’s University, Suwon-si, Gyeonggi-do, Korea ]
• In-Sook An [ 안인숙 | GeneCellPharm Corporation, Seoul, Korea ]
• Yong-Sam Kim [ 김영삼 | Department of Image Industry, Graduate School of Engineering, Konkuk University, Seoul, Korea ] Corresponding author

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