Earticle

초록

영어

The objectives of this study were: (1) to determine the impacts of high fat diet-induced obesity on mitochondrial function and insulin resistance in aged skeletal muscle and (2) to determine the effects of exercise training on mitochondrial dysfunction and insulin resistance induced by obesity and aging in skeletal muscle. Sprague-Dawley rats were randomly divided into four groups: 4-month young control (YC), 20-month old control (OC), old high fat diet group (OH), and old high fat diet and exercise group (OH+EX). After obesity was induced by 7 weeks of 60% high fat feeding in old groups, treadmill exercise training was performed 6 times a week for 7 weeks. Mitochondrial function (e.g., H2O2 emission, Ca2+ retention capacity) was measured in permeabilized skeletal muscle (e.g., soleus, and white gastrocnemius), and oral glucose tolerance test (OGTT) and Western immunoblot analysis were performed to determine insulin resistance. Mitochondrial H2O2 emission was increased in white gastrocnemius (type IIb muscle) of OC and OH compared with YC. However, exercise training attenuated mitochondrial H2O2 emission in OH+EX compared with OC and OH. Mitochondrial Ca2+ retention capacity was not significantly altered between groups in soleus (type I muscle). However, in white gastrocnemius, mitochondrial Ca2+ retention capacity was reduced in OC and OH compared with YC and, conversely improved in OH+EX compared with OC and OH. Insulin resistance was also increased in OC and OH compared with YC. By contrast, insulin resistance was attenuated in OH+EX compared with OC and OH. In conclusion, our data reported that high fat diet increased mitochondrial H2O2 emission, reduced mitochondrial Ca2+ retention capacity, and induced insulin resistance in aged skeletal muscle. However, exercise training reduced mitochondrial H2O2 emission, improved mitochondrial Ca2+ retention capacity, and attenuated insulin resistance in aged and obese skeletal muscle, suggesting that exercise training plays a protective role in aging and obesity (i.e., sarcopenic obesity)-induced mitochondrial dysfunction and insulin resistance in the rat skeletal muscle.

저자

• Jun-Won Heo [ Department of Kinesiology, Inha University, Incheon, Korea, WCSL, Inha University, Incheon, Korea ]
• Mi-Hyun No [ Department of Kinesiology, Inha University, Incheon, Korea, WCSL, Inha University, Incheon, Korea ]
• Han-Sam Cho [ Department of Kinesiology, Inha University, Incheon, Korea ]
• Tae-Woon Kim [ Department of Physiology, Kyung Hee University, Seoul, Korea ]
• Jin-Hwan Yoon [ Department of Kinesiology, Hannam University, Daejeon, Korea ]
• Dong-Ho Park [ Department of Kinesiology, Inha University, Incheon, Korea, WCSL, Inha University, Incheon, Korea ]
• Ju-Hee Kang [ WCSL, Inha University, Incheon, Korea, Department of Kinesiology, Hannam University, Daejeon, Korea ]
• Hyo-Bum Kwak [ Department of Kinesiology, Inha University, Incheon, Korea, WCSL, Inha University, Incheon, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국운동재활학회 학술대회

• 간기

  부정기

• 수록기간

  2011~2026

• 십진분류

  KDC 517 DDC 613