Earticle

초록

영어

Purpose: The purpose of the present study was to explore the possibility of using sinapic acid as a skin anti-aging cosmetic by demonstrating its antioxidant and anti-inflammatory effects in keratinocytes damaged by ultraviolet (UV) irradiation. Methods: The effects of sinapic acid against oxidative stress were examined in a UVB-induced aging model of human keratinocyte HaCaT cells. The water-soluble tetrazolium salt (WST-1) assay was employed to examine protective effects against UVB. Changes in the concentration of reactive oxygen species (ROS) produced by UVB were measured using 2’,7’-dichlorofluorescin diacetate (DCF-DA). Expression of genes associated with anti-oxidant and anti-inflammatory effects were confirmed using quantitative real-time polymerase chain reaction (qRT-PCR). Results: Sinapic acid protected cells from UVB-induced cytotoxicity and reduced ROS produced by UVB irradiation. Expression of genes associated with antioxidant effects including, superoxide dismutase 1 (SOD1 ), catalase (CAT), glutathione peroxidase 1 (GPX1 ), nuclear factor (erythroid-derived 2)-like 2 (NRF2 ), and heme oxygenase 1 (HO1 ), increased and lipid peroxidation decreased in a dose-dependent manner. The expression of cyclooxygenase 2 (COX2 ), interleukin 6 (IL6 ), and interleukin 8 (IL8 ) genes, which are associated with inflammation, also decreased in a dose-dependent manner. Conclusion: These results suggest that sinapic acid has potential as an anti-aging cosmetic ingredient with anti-oxidant, anti-inflammatory properties against UVB irradiation.

한국어

목적: 본 연구는 인간각질형성세포(HaCaT)에서 자외선(UV) 손상에 대한 항산화 및 항염증 효과를 확인하여 피부노화 예방 화장품 소재로서 sinapic acid의 가능성을 확인하고자 한다. 방법: Sinapic acid를 농도별로 HaCaT에 처리 후 UVB로 산화적 스트레스를 유도하였다. UVB에 대한 sinapic acid의 세포보호효과를 확인하기 위하여 water-soluble tetrazolium salt (WST-1)를 이용하였으 며, UVB에 의하여 생성된 활성산소종(reactive oxygen species, ROS)의 농도변화는 2’,7’-dichlorofluorescin diacetate (DCF-DA)를 사용하여 측정하였다. 항산화 및 염증과 관련된 유전자 발현은 quantitative real-time polymerase chain reaction (qRT-PCR)을 통 해 확인하였다. 결과: Sinapic acid는 UVB에 의한 세포독성으로부터 세포를 보호하고, ROS의 생성을 감소시킨다. 항산화와 관련 한 유전자인 superoxide dismutase 1 (SOD1), catalase (CAT), glutathione peroxidase 1 (GPX1), nuclear factor (erythroid-derived 2)-like 2 (NRF2) 및 heme oxygenase 1 (HO1)의 발현을 증가하게 하였고, 지질 과산화는 농도 의존적으로 감소하였다. 또한, 염증 과 관련이 있는 cyclooxygenase 2 (COX2), interleukin 6 (IL6)와 interleukin 8 (IL8)의 유전자 발현은 농도 의존적으로 감소하였다. 결론: 본 연구를 통하여 sinapic acid가 UVB에 대하여 항산화 및 항염증의 효과가 있음을 확인하였으며 이는 노화예방 화장품 성분 으로서의 가능성이 있을 것으로 사료된다.

중국어

目的: 探讨芥子酸对UVB诱导损伤的人角质形成细胞的抗氧化以及抗炎功效，确认芥子酸作为抗衰老化妆品原料的可 行性。方法: 对HaCaT细胞用不同浓度的芥子酸处理后，利用UVB进行氧化应激。为确认芥子酸的细胞保护作用，利用 water-soluble tetrazolium salt (WST-1)方法来评价；为确认由UVB诱导产生的活性氧 (reactive oxygen species, ROS)的 浓度变化，利用2’,7’-dichlorofluorescin diacetate (DCF-DA)来进行测定；为确认与抗氧化和抗炎相关的遗传因子的表达 程度，利用quantitative real-time polymerase chain reaction (qRT-PCR)方法来测定。结果: 芥子酸由UVB产生的细胞 毒性中保护细胞，并减少ROS的生成。芥子酸增加与抗氧化相关的遗传因子superoxide dismutase 1 (SOD1 )、catalase (CAT )、glutathione peroxidase 1 (GPX1) 、nuclear factor (erythroid-derived 2)-like 2 (NRF2 )以及heme oxygenase 1 (HO1 )等的表达程度，并以浓度依赖性方式减少脂质过氧化。此外，与炎症相关的遗传因子cyclooxygenase 2 (COX2 )、 interleukin 6 (IL6 )以及interleukin 8 (IL8 )的表达以浓度依赖性方式减少。结论: 从以上的研究结果，芥子酸对UVB具有抗 氧化和抗炎作用，因此作为预防衰老的化妆品原料充分具有可行性。

목차

Abstract
 Introduction
 Methods
  1. 세포배양
  2. 시료처리 및 자외선 조사
  3. 세포 생존율 측정
  4. 세포 내 ROS 정량 분석
  5. Quantitative real-time polymerase chain reaction (qRT-PCR)
  6. Lipid peroxidation 변화량 분석
  7. 통계분석
 Results and Discussion
  1. Sinapic acid의 세포 생존율 분석
  2. Sinapic acid의 ROS 제거 효능 분석
  3. Sinapic acid의 항산화 유전자 발현분석
  4. Sinapic acid의 lipid peroxidation 변화량 분석
  5. Sinapic acid의 항염증 유전자 발현분석
 Conclusion
 Acknowledgements
 References
 국문초록
 中文摘要

키워드

항산화 항염증 자외선 B 인간각질형성세포 Sinapic acid Anti-oxidant Anti-inflammation UVB HaCaT Sinapic acid UVB 芥子酸 抗氧化 抗炎 人角质形成细胞

저자

• Kye Hwa Lim [ 임계화 | Department of Beauty Care, Sangji Youngseo College, Wonju-si, Gangwon-do, Korea ]
• Jung-Eun Ku [ 구정은 | Department of Cosmetology, Kyung-In Women’s University, Incheon, Korea ]
• Sung-Ja Rhie [ 이승자 | Department of Beauty Care Design, Halla University, Wonju-si, Gangwon-do, Korea ]
• Ji Young Ryu [ 류지영 | Department of Beauty Care Design, Halla University, Wonju-si, Gangwon-do, Korea ]
• Seunghee Bae [ 배승희 | Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul, Korea ]
• Young-Sam Kim [ 김영삼 | Department of Image Industry, Graduate School of Engineering, Konkuk University, Seoul, Korea ] Corresponding author

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