Earticle

초록

영어

As fullerene-based compound application rapidly increased in health industry, the need of toxicological and biological effects is in demand. Here, we investigate the potential antioxidant properties of mineral-rich shungite and less mineral shungite in olive oil, fullerene-containing compound, against H2O2-induced oxidative stress in HaCaT cells. The composition of the less mineral shFig. 5. Inflammatory cytokines mediate H2O2-Induced Oxidative Stress. The concentration of (A) TNF-α, (B) IL-1β, (C) IL-10 cytokine was determined using ELISA after pure fullerene, mineral-rich shungite, and mineral-less shungite treatment.ungite is 94.79% atomic carbon analyzed by EDX X-ray spectrophotometer. Cell viability was significantly decreased by the exposure of mineral-rich shungite, mineral-less shungite, and pure fullerene C60 (25, 50, 100, and 200 μg/mL) for 24 hrs in the cultured HaCaT cells. Mineral-rich and less-mineral shungite compared to pure fullerene did not induce toxicity in vitro, therefore natural mineral shungite is safer to use for further investigation of its antioxidant property. Intracellular reduced glutathione (GSH) and catalase level were also decreased compared to the level of the non-treated control group during the exposure period, while the level of super oxide dismutase (SOD) did not have significant change. Intracellular cytokine levels of IL-10, IL-1β and TNF-α were increased suggesting an induced inflammatory response by shungite. This study suggests that natural mineral shungite could be a novel antioxidant and could provide a new insight for oxidative and inflammatory related disease treatment.

목차

ABSTRACT
 Introduction
 Materials and Methods
  1. Cell culture
  2. Preparation of pure fullerene, mineral- rich shungite, mineral-less shungite
  3. Induction of oxidative stress
  4. Cell Viability
  5. Determination of ROS Generation
  6. Endogenous Enzyme Activity: Glutathione Peroxidase Activity
  7. Superoxide dismutase (SOD) Assay
  8. Catalase Assay
  9. Enzyme-Linked Immunosorbent Assay(ELISA)
  10. Data management and Analysis.
 Results
  1. Effect of shungite in HaCaT cell viability
  2. Intracellular ROS mediates H2O2-induced oxidative stress in shungite-treated cells
  3. Endogenous enzyme activities mediates H2O2-induced oxidative stress
  4. Inflammatory cytokines mediate H2O2-induced oxidative stress.
 Discussion
 References

키워드

저자

• Ma. Easter Joy Sajo [ Department of Environmental Medical Biology ]
• Cheol-Su Kim [ Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea ]
• Kim Soo-Ki [ Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea ]
• Kyu-Jae Lee [ Department of Environmental Medical Biology, Institute for Poverty Alleviation and International Development, Yonsei University, Wonju, Gangwon 26493, Republic of Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국물학회지 [Korean Journal of Waters]

• 간기

  부정기

• pISSN

  2233-4653

• 수록기간

  2010~2021

• 십진분류

  KDC 539 DDC 628