Earticle

초록

영어

Purpose: 6-Shogaol is one of the spicy flavor ingredients of ginger and hydrolysis product of gingerols. The purpose of this study is to investigate the potential of 6-shogaol as a natural cosmetic raw material by examining cell protection and inflammation inhibitory activity in human dermal fibroblasts (HDFs). Methods: To verify the cytoprotective effects of 6-shogaol, cell viability was measured by water-soluble tetrazolium salt (WST-1) assay. To verify the anti-inflammatory effects of 6-shogaol, we performed nuclear factor kappa-light-chain-enhancer of activated B cells (NFΚB) promoter luciferase assay to check the expression of NFΚ B. Additionally, the expression of inflammation related gene such as cyclooxygenase 2 (COX2), receptor for advanced glycation end product (RAGE), tumor necrosis factor alpha (TNFΑ), interleukin 6 (IL6), and interleukin 8 (IL8) mRNA was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Results: 6-Shogaol showed no toxicity in HDFs at concentrations of 5, 10, 20, and 40 μM, respectively and the cell viability was increased in a dose dependent manner. qRT-PCR analysis showed 6- shogaol treatment downregulated the expression of NFΚB, COX2, RAGE, TNFΑ, IL6, and IL8 in a dose dependent manner, resulting that 6-shogaol leads to protective activities against inflammatory responses in HDFs. Conclusion: As mentioned above, 6-shogaol restored cell viability and decreased the expression of inflammatory factors in a dose dependent manner. Consequently, these results suggest the possibility of 6-shogaol as cosmetic material preventing skin aging, through identified functions on cytoprotection and anti-inflammation.

한국어

목적: 6-Shogaol은 생강(Zingiber officinale)의 매운맛을 내는 성분인 gingerols의 가수분해 산물로서, 본 연구에서는 6-shogaol 의 인간진피섬유아세포에서의 세포보호와 염증억제 효능을 검증함으로써 천연유래 화장품 원료로서의 사용가능성을 알아보고 자 한다. 방법: 6-Shogaol의 세포보호 효과를 검증하기 위하여, water-soluble tetrazolium salt (WST-1) assay를 이용하여 세포 생존율을 측정하였다. 6-Shogaol의 항염증 효과를 검증하기 위하여, nuclear factor kappa-light-chain-enhancer of activated B cells (NFΚB) promoter luciferase assay를 이용하여 염증반응 전사인자인 NFΚB의 발현을 측정하였으며 염증관련 유전자 들인 cyclooxygenase 2 (COX2), receptor for advanced glycation end product (RAGE), tumor necrosis factor alpha (TNFΑ), interleukin 6 (IL6), interleukin 8 (IL8) 발현량을 quantitative real-time polymerase chain reaction (qRT-PCR)를 이용하여 측정 하였다. 결과: 6-Shogaol은 5, 10, 20, 40 μM 각각의 농도에서 인간진피섬유아세포에 대한 독성을 보이지 않았으며 농도의존적 으로 10 J/cm²의 ultraviolet A (UVA)에 의해 손상된 인간진피섬유아세포의 생존율을 회복시켰다. 대표적인 염증반응 전사인자인 NFΚB의 promoter 활성이 6-shogaol에 의해 농도의존적으로 감소되었으며, 염증유발 유전자인 COX2, RAGE mRNA와 각종 염 증관련 사이토카인인 TNFΑ, IL6, IL8 mRNA의 발현도 6-shogaol에 의해 농도의존적으로 감소되었다. 결론: 6-Shogaol은 세포 보호 및 항염증에 대한 확인된 효능을 통해 피부노화를 예방하는 화장품 원료로서의 가능성을 보여줄 수 있으리라 사된료다.

중국어

目的: 6-Shogaol是生姜 (Zingiber officinale ) 的辛辣成分gingerols的水解产物。探讨6-shogaol对人皮肤成纤维细胞 的细胞保护和抗炎功效，从而鉴定6-shogaol作为天然化妆品原料的应用可行性。方法: 为确认6-Shogaol的细胞保 护效果，采用water-soluble tetrazolium salt (WST-1) 法测定细胞生存率实验。为验证6-Shogaol的抗炎功效，采用 nuclear factor kappa-light-chain-enhancer of activated B cells (NFΚB ) promoter luciferase assay测定炎症反应转 录因子NFΚB 的表达，以及采用 quantitative real-time polymerase chain reaction (qRT-PCR) 法确定炎症相关遗传因 子 cyclooxygenase 2 (COX2 )，receptor for advanced glycation end product (RAGE )，tumor necrosis factor alpha (TNFΑ )，interleukin 6 (IL6 )，interleukin 8 (IL8 ) 的表达量。结果: 6-Shogaol在5、10、20、40 μM的浓度下，对人皮 肤成纤维细胞没有毒性。6-Shogaol对被10 J/cm² ultraviolet A (UVA) 损伤的人皮肤成纤维细胞的生存率呈浓度依赖性 恢复。代表性的炎症转录因子NFΚB 的promoter活性被6-shogaol呈浓度依赖性降低。炎症诱发遗传因子COX2 ，RAGE mRNA和各种炎症相关细胞因子TNFΑ 、IL6 、IL8 mRNA的表达被6-shogaol呈浓度依赖性降低。结论: 通过以上研究确 认了6-Shogaol具有细胞保护和抗炎功效，因此作为预防抗衰老的化妆品原料充分具有可行性。

목차

Abstract
 Introduction
 Methods
  1. 세포배양 및 시료처리
  2. Cell viability 측정
  3. RNA 추출과 cDNA 제조
  4. qRT-PCR
  5. NFKB luciferase assay
  6. 통계처리
 Results and Discussion
  1. 6-Shogaol 및 UVA의 세포독성
  2. 6-Shogaol의 세포보호 효과
  3. NFΚB 의 promoter 활성억제 효과
  4. COX2 mRNA, RAGE mRNA 발현 변화
  5. TNFΑ mRNA, IL6 mRNA, IL8 mRNA 발현 변화
 Conclusion
 References
 국문초록
 참고문헌
 中文摘要

키워드

인간진피섬유아세포 세포보호 항염증 화장품 Human dermal fibroblast Cytoprotection Anti-inflammation 6-Shogaol Cosmetic 6-Shogaol 6-Shogaol 人皮肤成纤维细胞 细胞保护 抗炎症 化妆品

저자

• Na-Kyeong Lee [ 이나경 | Department of Cosmetics, JEI University, Incheon, Korea ]
• Jung-Eun Ku [ 구정은 | Department of Cosmetology, Kyung-In Women’s University, Incheon, Korea ]
• Hyo Sun Han [ 한효선 | Korea Institute of Dermatological Sciences, Seoul, Korea, Department of Biological Engineering, Konkuk University, Seoul, Korea ] Corresponding author

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