Earticle

초록

영어

Transplantation of bone marrow stromal cells (BMSCs) is regarded as a promising candidate for the spinal cord injury (SCI). In the present study, we investigated whether treadmill exercise potentiate the effect of BM-SCs transplantation on the functional recovery in the SCI rats. The spi-nal cord contusion injury applied at the T9–T10 level using the impactor. Cultured BMSCs were transplanted into the lesion at 1 week after SCI induction. Treadmill exercise was conducted for 6 weeks. Basso-Beat-tie-Bresnahan (BBB) scale for locomotor function was determined. Sprouting axons in the lesion cavity were detected by immunofluores-cence staining for neurofilament-200. Brain-derived neurotrophic factor (BDNF) and synapsin-I expressions were analyzed using western blot-ting. BMSCs transplantation improved BBB score and increased ex-pressions of neurofilament-200, BDNF, and synapsin-I in the SCI rats. Treadmill exercise potentiated the improving effect of BMSCs trans-plantation on BBB score in the SCI rats. This potentiating effect of treadmill exercise could be ascribed to the enhancement of BDNF ex-pression in the SCI rats.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Animals and treatments
  Spinal cord contusion injury
  BMSCs culture and transplantation
  Treadmill exercise protocol
  BBB locomotor scale
  Tissue preparation
  Immunofluorescence
  Western blot analysis
  Data analysis
 RESULTS
  BBB score
  Axonal sprouting within the cavity
  BDNF expression
  Synapsin-I expression
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• You-Mi Kim [ Sports Science Research Institution, Korea National Sport University, Seoul, Korea ]
• Tae-Beom Seo [ Division of Sports Science and Engineering, Korea Institute of Sports Science, Seoul, Korea ]
• Chang-Ju Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Eun-Sang Ji [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613