Earticle

초록

영어

Purpose: The anti-oxidant activity of active ingredients extracted from Rosa multiflora root was evaluated and effects of cell proliferation and growth factor expression were confirmed. Methods: Total polyphenol and flavonoid content of the active ingredients extracted from Rosa multiflora root was measured in various ways, as were 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and nitrite scavenging activity. Further, the cell proliferation effects for human hair follicle dermal papilla cell (HFDPC) were measured using thiazolyl blue tetrazolium bromide (MTT) assay, as were the effects of promoting the expression of growth factors such as platelet derived growth factor subunit B (PDGFB), keratinocyte growth factor (KGF), insulin like growth factor 1 (IGF1), vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and epidermal growth factor (EGF) by Western blot. Results: The total polyphenol content of six kinds of Rosa multiflora root extracts was the highest in supercritical extracts (101.925 mg/g) and the total flavonoid content was the highest in supercritical extracts (14.365 mg/g). DPPH scavenging activity was the highest in methanol-sonication extracts (98.24%) and nitrite scavenging activity was the highest in supercritical extracts (55.86%). HFDPC was treated with 10, 100, and 500 μg/mL of Rosa multiflora root methanol-sonication extracts to determine cytotoxicity. The cell growth rate increased with increasing concentrations. The effects of the expression of growth factor protein were 135% greater in IGF1 and 126% greater in PDGFB. Conclusion: Rosa multiflora root extracts had no cytotoxicity but had excellent anti-oxidant ability. In addition, because the effects of promoting the expression of IGF1 and PDGFB were significant, it was confirmed that Rosa multiflora root extracts could be used as an antioxidant, functional cosmetic material, and as a natural material for hair restorer and a hair-growth promotion agent.

한국어

목적: 찔레나무(Rosa multiflora) 뿌리에서 유효성분을 추출하여 항산화 활성 평가, 세포증식효과 및 성장인자 발현촉진효과를 확인하여 천연성분을 이용한 기능성 화장품 및 육모제 소재로서의 활용 가능성을 탐색하였다. 방법: 찔레나무 뿌리에서 다양한 방법으로 유효성분을 추출하여 총 폴리페놀 및 플라보노이드 함량을 측정하였고, DPPH 라디칼 소거능과 아질산염 소거능을 측정하였다. MTT assay로 인간모유두세포에 대하여 세포 독성 효과를 측정하였으며, 웨스턴블롯을 통해 모발성장인자인 PDGFB, KGF, IGF1, VEGF, HGF, EGF의 발현촉진효과를 측정하였다. 결과: 찔레뿌리 추출물 6종의 총 폴리페놀 함량은 초임계 추출물(101.925 mg/g), 총 플라보노이드 함량은 초임계 추출물(14.365 mg/g)에서 가장 높게 나타났다. DPPH 소거능은 메탄올-초음파 추출물(98.24%)에서, 아질산염 소거능은 초임계 추출물(55.86%)에서 가장 높게 나타났다. 인간모유두세포에 찔레뿌리 메탄올-초음파 추출물을 10, 100, 500 μg/mL의 농도별로 처리하여 세포 독성을 확인한 결과, 처리농도 범위 내에서 세포 독성은 관찰되지 않았으며. 농도가 증가할수록 세포 생육도가 높았다. 웨스턴블롯을 통한 성장인자 단백질의 발현효과는 대조군 대비 IGF1은 135%, PDGFB은126%로 발현촉진효과가 높게 나타났다. 결론: 찔레뿌리 추출물은 독성이 없고 항산화능이 우수하였으며, IGF1과 PDGFB에 대한발현촉진효과가 높게 나타나 항산화제, 기능성화장품, 육모제 및 발모촉진제의 천연소재로서의 활용 가능성을 확인하였다.

중국어

目的: 在蔷薇树根中提取有效成分，评价其提取物的抗氧化活性、细胞增殖效果及成长因子表达促进效果，探讨作为功 能性天然化妆品及育发剂原料的应用可行性。方法: 在蔷薇树根中利用多种方法提取有效成分，分别测定总多酚含量和 类黄酮含量，并测定DPPH自由基消除能力和亚硝酸盐的消除能力。对人毛囊真皮乳头细胞利用MTT assay法来测定细胞 毒性，应用Western blot测定PDGFB、KGF、IGF1、VEGF、HGF、EGF等生长因子的表达促进效果。结果: 在6种蔷薇树 根提取物的总多酚含量和类黄酮含量中，其中超临界萃取物以总多酚为101.925 mg/g，类黄酮含量以14.365 mg/g显示最 高。DPPH消除能力在甲醇-超音波提取物中以98.24%，亚硝酸盐消除能力在超临界萃取物中以55.86%显示最高。对人毛 囊真皮乳头细胞，利用蔷薇树根甲醇-超音波提取物分别以不同浓度10, 100, 500 μg/mL进行处理，测定细胞毒性结果显 示：在处理浓度范围内没有观察到细胞毒性，浓度越高，细胞生长速率越高。利用Western blot检测细胞成长因子的蛋白 质表达结果显示：与对照群相比IGF1以135%，PDGFB以126%显现高效果。结论: 蔷薇树根提取物没有毒性，具有优秀的 抗氧化能力，对IGF1和PDGFB的表达具有高促进效果。因此作为抗氧化剂、功能性化妆品，育发剂以及发毛促进剂的天 然原料充分具有可行性。

목차

Abstract
 Introduction
 Methods
  1. 실험재료 및 시약
  2. 실험방법
  3. 통계처리
 Results
  1. 추출 수율
  2. 총 폴리페놀 함량
  3. 총 플라보노이드 함량
  4. DPPH 라디칼 소거능
  5. 아질산염 소거능
  6. HFDPC에 대한 in vitro 세포 독성 평가
  7. 모발성장인자 단백질 발현촉진효과
 Discussion
 Conclusion
 References
 국문초록
 中文摘要

키워드

찔레나무 인간모유두세포 웨스턴블롯 성장인자 MTT assay Rosa multiflora Human hair follicle dermal papilla cells Western blot Growth factor MTT assay Western blot MTT assay 蔷薇树 人毛囊真皮乳头细胞 成长因子

저자

• Hyeon Sook Hwang [ 황현숙 | Department of Complementary and Alternative Medicine, Chosun University, Gwangju, Korea ]
• Tae Hyun Hwang [ 황태현 | Department of Complementary and Alternative Medicine, Chosun University, Gwangju, Korea ]
• Ae Ja Pyo [ 표애자 | Department of Complementary and Alternative Medicine, Chosun University, Gwangju, Korea ]
• Eun Hwa Ju [ 주은화 | Department of Complementary and Alternative Medicine, Chosun University, Gwangju, Korea ] Corresponding author

참고문헌

발행기관

간행물