Earticle

초록

영어

Purpose: This study aimed to analyze the applicability of extracts of Opuntia humifusa fruit (OHF) as functional cosmetic ingredients. Methods: Total polyphenol and flavonoid contents were measured, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical elimination, melanogenesis inhibition, microphthalmia-associated transcription factor (MITF), and tyrosinase expression were evaluated. Furthermore, inhibitory effects of matrix metalloproteinase 1 (MMP1), phosphorylated extracellular signal-related kinase (p-ERK), and phosphorylated c-Jun N-terminal kinase (p-JNK) were evaluated. Results: DPPH radical elimination and high polyphenol content were confirmed in extracts of OHF. As a result of a cytotoxicity experiment with the extracts of OHF on B16F10 and human dermal fibroblast cells, no cytotoxicity effects was observed at any of concentrations tested in both cells. The effects of melanogenesis inhibition even at a low concentration was established. In addition, a slight inhibitory effects on tyrosinase and MITF expression, reduction in MMP1 expression, and inhibition of phosphorylation of JNK were also confirmed. Conclusion: The extracts of OHF have excellent anti-oxidant effects, thus the extracts are valuable as ingredients in various functional cosmetics with whitening and wrinkle-improving effects.

한국어

목적: 천년초 열매 추출물(Opuntia humifusa fruit extract)의 기능성 화장품 소재로서의 활용 가능성을 확인하고자 하였다. 방법: 천년초 열매 추출물의 항노화 및 미백 효능을 확인하기 위하여 천년초 열매 추출물의 총 폴리페놀 함량, 총 플라보노 이드 함량, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical 소거능, 멜라닌 생성 억제능, microphthalmia-associated transcription factor (MITF)와 tyrosinase 발현량, matrix metalloproteinase 1 (MMP1), phosphorylated extracellular signal-related kinase (p-ERK)와 phosphorylated c-Jun N-terminal kinase (p-JNK) 발현량 억제 효과를 측정하였다. 결과: 천년초 열매 추출물에서 천년초 줄기 추출물 대비 높은 폴리페놀 함량과 우수한 DPPH radical 소거능을 확인하였으며, B16F10, human dermal fibroblast 세포에서 천년초 열매 추출물에 대한 세포 독성 실험을 진행한 결과 모든 처리 농도에서 세포 독성이 나타나지 않았다. 저농도에서도 멜라닌 생성 억제 효과를 확인하였으며, tyrosinase와 MITF 발현량의 미약한 억제 효과를 확인하였다. 또한 MMP1 발현 감소와 JNK의 인산화가 억제되는 것을 확인하였다. 결론: 천년초 열매 추출물은 항산화 효과가 우수하고, 미백 개선 및 주름 개선 효과를 가지는 다양한 기능성 화장품 소재로서의 가치가 있는 것으로 사료 된다.

중국어

目的: 探索千年草果子提取物作为功效性化妆品原料的活用可行性。方法: 确认千年草果子提取物的抗老化以及美白功 效，测定提取物的总多酚含量和类黄酮的含量，并对1,1-diphenyl-2-picrylhydrazyl (DPPH)自由基消除能力，抑制黑色素 生成，microphthalmia-associated transcription factor (MITF)及酪氨酸酶的表达， matrix metalloproteinase 1 (MMP1)、 phosphorylated extracellular signal-related kinase (p-ERK)、 phosphorylated c-Jun N-terminal kinase (p-JNK)蛋白表达的 抑制效果进行了评价。结果: 千年草果子的提取物比茎提取物DPPH自由基消除能力优秀，总多酚含量也高。在 B16F10, human dermal fibroblast 细胞中对千年草果子提取物进行细胞毒性试验，结果显示，在提取物所有处理浓度范围内，无明 显细胞毒性。即使在低浓度也能抑制黑色素的形成，对酪氨酸酶和MITF的表达也具有微小的抑制作用。此外，也确认了 MMP1的表达明显减少以及抑制JNK的磷酸化。结论: 千年草果子提取物具有优秀的抗氧化作用，因此作为美白及改善皱纹 的功效性化妆品原料充分具有价值。

목차

Abstract
 Introduction
 Methods
  1. 실험 재료
  2. 항산화 효과 측정
  3. Neutral red assay를 이용한 세포 독성 측정
  4. 멜라닌 생성 억제능 측정
  5. MMP1 발현 억제능 측정
  6. Western blotting
  7. 통계 처리
 Results and Discussion
  1. 항산화 효과 측정 결과
  2. 항멜라닌 효과 측정 결과
  3. 항노화 효과 측정 결과
 Conclusion
 References
 국문초록
 참고문헌
 中文摘要

키워드

천년초 항산화 폴리페놀 멜라닌 항노화 Opuntia humifusa Anti-oxidant effects Polyphenol Melanin Anti-aging effects 千年草 抗氧化 多酚 黑色素 抗氧化

저자

• Min-Jung Ha [ 하민정 | Department of Biological Engineering, Konkuk University, Seoul, Korea ]
• Seon-Hee You [ 유선희 | Department of Biological Engineering, Konkuk University, Seoul, Korea ] Corresponding author

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