Min-Woo Hyun, So-Jin Yeob, Hye-Sung Park, Jae-Han Cho, Kang-Hyo Lee, Jae-Gu Han
언어
영어(ENG)
URL
https://www.earticle.net/Article/A289471
원문정보
초록
영어
Isaria farinosa (Hypocreales, Ascomycota) is a cosmopolitan entomopathogenic fungus affecting a wide range of arthropod hosts. It has mainly been studied as a insecticidal agent to control the agricultural pests. To investigate the useful secondary metabolite(SM) genes in Isaria farinosa C1012 strain, de novo assembly and genome mining were carried out. A whole genome sequencing with PacBio RSII system generated NGS reads greater than 4Gb, which were assembled into 16 contigs using FALCON program. The total size of genome was 33.36Mb. The N50 and N90 were 6,686,213 and 1,912,865bp, respectively. The assembled genome data was analyzed with antiSMASH3 program with a default setting to localize the gene region responsible for synthesizing SMs, such as non-ribosomal peptide synthetases (NRPS) and polyketide synthase (PKS). In this study, we predicted 16 NRPS, 13 PKS, and 9 PKS-NRPS hybrid gene clusters in I. farinosa genome.
저자
Min-Woo Hyun [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of Korea ]
So-Jin Yeob [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of Korea ]
Hye-Sung Park [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of Korea ]
Jae-Han Cho [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of Korea ]
Kang-Hyo Lee [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of Korea ]
Jae-Gu Han [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of Korea ]