Earticle

초록

영어

Purpose: This study was conducted to evaluate anti-oxidant and anti-melanogenic activities of pine cone extract from Pinus densiflora. Methods: Pine cone extract used in this experiment was extracted with methanol at room temperature for 24 h. Anti-oxidant activities were measured by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and nitro blue tetrazolium chloride (NBT) assay. Moreover, the inhibitory effects of pine cone methanol extract on tyrosinase and melanin synthesis in murine B16-F10 melanoma cells were studied. In addition, cell viability was investigated by MTT assay. Results: The results showed that the pine cone methanol extract strongly reduced the DPPH free radical activity (IC50=9.57±1.24 μg/mL). However, its activity was lower than the activity of L-ascorbic acid (positive control, IC50=1.28±0.03 μg/mL). In NBT assay, the pine cone methanol extract exhibited more superoxide anion radical scavenging activity (IC50=3.33±0.28 μg/mL), compared with L-ascorbic acid (IC50=73.25±1.02 μg/mL). When treated with 3,4-Dihydroxy-L-phenylalanine (L-DOPA) as the substrate of tyrosinase, the extract inhibited the tyrosinase activity with an IC50 value of 106.15±1.69 μg/mL, lower effect than that of kojic acid (IC50=3.12±0.68 μg/mL). In murine B16-F10 cells, the pine cone methanol extract significantly suppressed melanogenesis in a concentration-dependent manner without cytotoxicity. Conclusion: The results indicated that pine cone extract could be potentially valuable as a new potent depigmentation agent.

한국어

목적: 본 논문의 목적은 솔방울 메탄올 추출물의 항산화 효과 및 항멜라닌 활성을 평가하는 것이다. 방법: 솔방울 메탄올추출물의 항산화 활성을 알아보기 위하여 2,2-Diphenyl-1-picrylhydrazyl (DPPH) 및 슈퍼옥사이드 음이온 라디칼 소거능(superoxide anion radical scavenging activity)을 측정하였을 뿐 아니라, 미백효능을 평가하기 위하여 티로시나아제에3,4-Dihydroxy-L-phenylalanine (L-DOPA)를 가하여 티로시나아제 활성 저해효능을 측정하였다. 또한 멜라닌 생합성 억제효과는 α-MSH를 가한 B16-F10 세포를 활용하여 확인하였고, Thiazolyl blue tetrazolium bromide (MTT)를 활용하여세포독성을 측정하였다. 결과: 솔방울 메탄올 추출물은 높은 항산화 활성을 가지고 있고, 농도의존적으로 티로시나아제를 억제하였을 뿐 아니라, B16-F10 세포에서 농도의존적으로 멜라닌 형성을 저해하였다. B16-F10 및 CCD-1064SK 세포에서 솔방울 메탄올 추출물은 세포독성을 나타내지 않았다. 결론: 솔방울 메탄올 추출물은 세포독성을 나타내지 않으며 멜라닌 형성을 저해하고 항산화 효과 또한 뛰어나므로 미백 및 항산화 소재로 충분히 활용될 수 있다고 사료된다.

중국어

目的: 研究赤松松果甲醇提取物的抗氧化及抗黑色素效果。方法: 为了评价赤松松果甲醇提取物的抗氧化效果，利用 2,2-Diphenyl-1-picrylhydrazyl (DPPH) 方法及超氧阴离子自由基消除能力(superoxide anion radical scavenging activity)来测定；为了评价美白效能，蘑菇酪氨酸酶中加入3,4-Dihydroxy-L-phenylalanine (L-DOPA)，测定酪氨酸酶 的活性抑制效能；添加了α-MSH 的B16-F10细胞来测定黑色素抑制效果；利用Thiazolyl blue tetrazolium bromide (MTT) 法测定细胞毒性。结果: 赤松松果甲醇提取物具有很高的抗氧化能力，不仅抑制蘑菇酪氨酸酶，而且在 B16-F10细胞中抑制黑色素的形成。随着赤松松果甲醇提取物的浓度的升高，蘑菇酪氨酸酶的抑制效果和抑制黑色素 的形成也随之增加。对B16-F10 和 CCD-1064SK细胞，赤松松果甲醇提取物不具有细胞毒性。结论: 赤松松果甲醇提 取物不具有细胞毒性，不仅抑制黑色素的形成，而且抗氧化效果也非常明显，因此作为美白及抗氧化原料充分具有可 行性。

목차

Abstract
 Introduction
 Methods
  1. 실험재료
  2. 시료준비
  3. 전자공여능 측정
  4. 활성산소 소거능 측정
  5. Tyrosinase 활성 억제 효과
  6. Melanin 생합성 억제 효과
  7. B16-F10 및 CCD-1064SK 세포독성 평가
  8. 통계분석
 Results and Discussion
  1. 전자공여능
  2. 활성산소 소거능
  3. Tyrosinase 활성 억제 효과
  4. Melanin 생합성 억제 효과
  5. B16-F10 및 CCD-1064SK 세포에서 솔방울 메탄올 추출물의 세포독성 평가
 Conclusion
 References
 국문초록
 참고문헌
 中文摘要

키워드

솔방울 메탄올 추출물 항산화 활성 항멜라닌 활성 세포독성 Pine cone Methanol extract Anti-oxidant activity Anti-melanogenic activity Cytotoxicity 赤松松果 甲醇提取物 抗氧化活性 抗黑色素活性 细胞毒性

저자

• Ah Reum Lee [ 이아름 | College of Korean Medicine, Daegu Haany University, Daegu, Korea ]
• Seong-Soo Roh [ 노성수 | College of Korean Medicine, Daegu Haany University, Daegu, Korea ]
• Eun-Sook Lee [ 이은숙 | Department of Herbal Skin Care & Cosmetology, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, Korea ]
• Yu-Hong Min [ 민유홍 | Department of Beauty Care, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, Korea ] Corresponding author

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