Earticle

초록

영어

Purpose: This study was conducted to provide data of the material development of functional cosmetics and hair growth products that could replace the drug therapies by extracting the active ingredients from Dendropanax morbifera (D. morbifera) leaf using the methanol-ultrasound extraction method. Methods: Total polyphenol and flavonoid contents of D. morbifera leaf methanol-ultrasound extract were measured. Also, the anti-oxidant capacity, nitrite scavenging ability, anti-microbial activity, and the minimum inhibitory concentration (MIC) were measured. Cytotoxicity of the extract on HFDPCs was examined by MTT assay at concentrations of 10, 100, and 500 μg/mL, respectively. Moreover, the promotive and inhibitory effects of growth factor expression were also confirmed through the western blotting. Results: Total polyphenol and flavonoid contents of D. morbifera leaf methanol-ultrasound extract were 74.082±7.180 mg/g and 97.360±2.235 mg/g, respectively. DPPH scavenging ability was 92.440±1.023%, which was very high, and the nitrite scavenging activity was 52.6±2.4%. Anti-microbial activities were investigated in the strains such as Propionibacterium acnes (P. acnes), Pityrosporum ovale (P. ovale), and Malassezia furfur (M. furfur). The MIC of P. acnes, P. ovale, and M. furfur were 17.3%, 21.6%, and 15.7%, respectively. Cytotoxicity was not observed within the range of concentrations (10, 100, and 500 μg/mL) of D. morbifera leaf methanol-ultrasound extract and the cell growth rate was increased according to the concentrations. In case of the growth factor expression by western blotting, high promotive effect was observed in IGF protein expression (156±8%) compared with the control group, while inhibitory effect was confirmed in TGFβ2 protein expression (78.3±10%). Conclusion: Therefore, it is possible to utilize D. morbifera leaf methanol-ultrasound extract as the material of functional cosmetics and hair growth products with anti-oxidant capacity.

한국어

목적: 본 연구는 황칠나무(Dendropanax morbifera) 잎에서 메탄올-초음파 추출법으로 유효성분을 추출하여 약물요법을 대체 할 수 있는 기능성 화장품 및 육모제 소재 개발에 자료를 제공하고자 하는 목적으로 수행하였다. 방법: 황칠나무 잎 메탄올-초음파 추출물의 총 폴리페놀 및 플라보노이드 함량을 측정하였고, 항산화능과 아질산염 소거능을 측정하였으며, 항균활성과 최소성장저해농도(minimum inhibitory concentration, MIC)를 측정하였다. HFDPC세포에 대하여 10, 100, 500 μg/mL 농도별로 처리하여 MTT assay를 통하여 세포독성을 평가하였으며, western blotting을 통한 성장인자 발현촉진 및 억제 효과를 측정하였다. 결과: 황칠나무 잎 메탄올-초음파 추출물의 총 폴리페놀 함량은 74.082±7.180 mg/g, 총 플라보노이드 함량은 97.360±2.235 mg/g로 나타났다. DPPH 소거능은 92.440±1.023%로 높은 효과를 보였으며, 아질산염 소거능은 52.6±2.4%를 보였다. Propionibacterium acnes (P. acnes), Pityrosporum ovale (P. ovale), Malassezia furfur (M. furfur) 균주에 대하여 항균활성이 나타났으며, 최소성장저해농도(MIC)는 P. acnes에서는 17.3%, P. ovale는 21.6%, M. furfur 에서는15.7% 농도로 나타났다. 인간모유두세포에 황칠나무 추출물을 10, 100, 500 μg/mL의 처리농도 범위 내에서세포독성은 관찰되지 않았으며, 농도가 증가할수록 세포생육도가 높았다. Western blotting을 통한 성장인자 단백질의 발현및 억제 효과는 대조군 대비 IGF는 156±8%로 발현촉진 효과가, TGFβ2는78.3±10%로 억제효과가 나타났다. 결론: 황칠나무 잎 메탄올-초음파 추출물은 항산화능을 가진 기능성 화장품 및 육모제의 소재로 활용가능성이 있는 것으로 사료된다.

중국어

目的: 利用甲醇-超音波提取法提取黄漆树(Dendropanax morbifera)叶的有效成分，研究其性能，为代替药物疗法的功效性 化妆品和育发剂原料提供研究资料。方法: 测定黄漆树叶的甲醇-超声波提取物的总多酚含量和类黄酮的含量，并检测抗氧 化能力、亚硝酸盐的消除能力、抗菌活性和最低抑菌浓度（minimum inhibitory concentration，MIC）。对人毛囊真皮乳头 细胞，用黄漆树叶提取物分别以不同浓度10，100，500 μg/mL进行处理，并用MTT assay进行测定，应用western blotting 检测生长因子表达的促进效果。结果: 黄漆树叶提取物的多酚含量为74.082±7.180，总类黄酮的含量为97.360±2.235 mg/g。 DPPH消除能力为92.440±1.023%，显示最高效果，而亚硝酸盐的消除能力为52.6±2.4%。黄漆树叶提取物对 Propionibacterium acnes (P. acnes)，Pityrosporum ovale (P. ovale)， Malassezia furfur (M. furfur) 菌具有抗菌效果，最低抑 菌浓度（MIC）依次为17.3%（P. acnes)，21.6%（P. ovale)，15.7% （M. furfur）。对人毛囊真皮乳头细胞用黄漆树叶提取物 分别以10，100，500 μg/mL浓度处理时，没有观察到细胞毒性，浓度越高，细胞生长速率越高。通过western blotting观察 生长因子蛋白的表达效果，结果显示与对照群相比IGF的促进效果为156±8%，TGFß2的抑制效果为78.3±10%。结论: 黄漆 树叶的甲醇-超声波提取物具有抗氧化能力，作为功效性化妆品和育发产品原料充分具有活用可行性。

목차

Abstract
 Introduction
 Methods
  1. 실험재료 및 시약
  2. 실험방법
  3. 통계처리
 Results and Discussion
  1. 총 폴리페놀 및 플라보노이드 함량
  2. DPPH radical 소거능
  3. 아질산염 소거능
  4. Paper disc 방법을 이용한 항균활성 측정
  5. 최소성장저해농도(MIC) 측정
  6. 세포독성평가 및 세포증식효과
  7. 모발성장인자 단백질발현 촉진 및 억제효과
 Conclusion
 References
 국문초록
 참고문헌
 中文摘要

키워드

황칠나무 인간모유두세포 성장인자단백질 Western blotting DPPH Dendropanax morbifera Human hair follicle dermal papilla cell Western blotting Growth factor protein DPPH Western blotting DPPH 黄漆树 人毛囊真皮乳头细胞 生长因子蛋白

저자

• Yun Mi Park [ 박윤미 | Department of Cosmetology Science, Graduate School of Nambu University, Gwangju, Korea ]
• Jin Seop Han [ 한진섭 | Department of Cosmetology Science, Graduate School of Nambu University, Gwangju, Korea ] Corresponding author

참고문헌

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