One of subtypes in ultraviolet (UV), UVB, has been reported that the most powerful factors causing photoaging and DNA lesions. Finding and developing both cost-effective and efficient materials are the urgent issues to protect the skin from UVB-induced damages. Alpha-mangostin (α-mangostin), the first xanthone isolated from Garcinia mangostana (mangosteen), has been studied for anti-inflammation and antioxidant properties. The purpose of this study to investigate effects of α-mangostin in HaCaT, keratinocyte against UVB radiation. To evaluate effects of α-mangostin upon UVB-induced cytotoxic damages, watersoluble tetrazolium salt (WST-1) assay, flow Cytometry analysis, qunatitative real-time PCR (qRT-PCR), and senescence associated β-galactosidase assay (SA-β-gal assay) were performed. Pretreatment of α-mangostin was verified protective effects on UVB-induced proliferative restrain, apoptotic cell death and senescence, in HaCaT keratinocytes. These results indicated the protective effects of α-mangostin against UVB, and suggest as a cosmeceutical ingredient.
목차
Abstract Ⅰ. 서론 Ⅱ. 연구방법 1. 세포배양 2. 시료처리 및 자외선 조사 3. 세포생존율 측정 4. Quantitative real-time PCR 5. 세포주기 측정 6. 세포노화 측정 7. 통계처리 Ⅲ. 연구결과 및 고찰 1. α-mangostin의 UVB에 의한 세포독성 보호효과 분석 2. α-mangostin의 UVB에 의한 세포자살 보호 효과 3. α-mangostin의 UVB에 의한 세포노화 보호 효과 Ⅳ. 결론 참고문헌