Earticle

초록

영어

Cocoa extracts contain various anti-oxidant which play as anti-skin aging agents. Procyanidin B1 is one of major ingredients in cocoa. However, in dermis, effects of Procyanidin B1 are not understood currently. Therefore, we investigated whether procyanidin B1 regulated extra-cellular matrix (ECM) and exerts its therapeutic action and its effect on the anti-wrinkle. We show firstly cytotoxicity of procyanidin B1. Under 100 μg/ml procyanidin B1 show no cytotoxicity in normal human dermal fibroblasts (nHDFs). In addition, we measured matrix metalloprotease 1 (MMP1) mRNA expression in procyanidin B1 exposed nHDFs. As shown results, procyanidin B1 repressed oxidative stress induced increase of MMP1 mRNA expression. And procyanidin B1 repressed activity of TPA responsive element (TRE) which is DNA binding site of AP-1 complex which consist c-Jun and c-Fos. JNK-c-Jun axis is implicated oxidative stress-mediated MMP1 mRNA regulation. In addition, JNK is activated using phosphorylation of JNK. Therefore, we shown phosphorylation in procyanidin B1-exposed nHDFs. As shown results, procyanidin B1 decreased oxidativemediated JNK phosphorylation of JNK. Overall, our results suggest procyanidin B1 is a potential cosmetic ingredient repressed antiaging and anti-wrinkle in skin using repression of oxidative-induced MMP1 expression.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 세포배양
  2. 시료
  3. Cell viability assay
  4. Quantitative real time polymerase chain reaction (qRT-PCR)
  5. TRE-LUC
  6. 통계처리
 Ⅲ. 연구결과 및 고찰
  1. nHDFs에서 procyanidin B1의 세포독성측정
  2. Procyanidin B1의 MMP1 mRNA 발현 억제 효과
  3. Procyanidin B1에 의한 AP-1의 전사 활성 변화 산화적 스트레스
  4. Procyanidin B1에 의한 JNK의 phosphorylation 변화
 Ⅳ. 결론
 참고문헌

키워드

저자

• 차화준 [ Hwa Jun Cha | 건국대학교 생물공학과 ]
• 김영주 [ Young-Joo Kim | 오산대학교 뷰티디자인계열 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646