In order to check the degree ofcontamination from the activities by space andtime in the school facility where manystudents and educational personnels spend most of their time, quantity of airbornebacteria was counted by indoor air qualityprocess test method of the Ministry ofEnvironment. According to the data providedby Environmental Management Corporation,PM-10 concentration of February 23, 2015 wasthe only harmful value which causedCAI(Comprehensive Air-quality Index) to be inmuch bad degree. Comparing the numbers ofbacteria in the morning when the school lifestarts and lunch break, the increase of 55% to200% was found on the hallway, while thecommissary(cafeteria) where students used tovisit the most showed the increase of 56% to1230%. According to the result of comparison between the outcome from the sequenceanalysis of 16S rRNA gene of the randomlyselected and purely cultured bacteria and theExTaxon Database, the bacteria wereidentified as Staphylococcus epidermidis(99%),Kocuria palustris(99%), and Bacillusaryabhattai(99%).
초록 Ⅰ. 서 론 Ⅱ. 재료 및 방법 2-1 대기오염도 분석 2-2 공기 중 미생물의 포집 3-3 공기 중 미생물의 순수배양 및 동정 Ⅲ. 결과 및 고찰 3-1 대기오염도 분석 3-2 부유세균의 균체수 3-3 16S rRNA gene 염기서열 분석 참고 문헌 Abstract