느타리버섯 수확후배지로부터 분리한 Bacillus subtilis CA105의 특성
Isolation and Characterization of Bacillus subtilis CA105 from Spent Mushroom (Pleurotus ostreatus) Substrates
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In order to isolate compost-promoting bacteria with high activity of cellulase and xylanase, spent mushroom substrates with sawdust were collected from mushroom cultivation farm, Jinju, Gyeongnam in Korea. Among of the isolates, one strain, designated CA105 was selected by agar diffusion method. The strain CA105 was identified as members of the Bacillus subtilis by biochemical characteristics using VITEK 2 system. Comparative 16S rRNA gene sequence analysis showed that isolate CA105 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus subtilis with 16S rRNA gene sequence similarity of 98.9%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, isolate CA105 was classified within the genus Bacillus subtilis, for which the name Bacillus subtilis CA105 is proposed. The cellulase and xylanase activity of B. subtilis CA105 was slightly increased according to bacterial population from exponential phase to stationary phase in growth curve for Bacillus sp. CA105.
목차
ABSTRACT 서론 재료 및 방법 느타리버섯 수확후배지에 우점하는 미생물 분리 분리균의 생리적 특성 및 생화학적 특성 조사 분리균의 16S rRNA 염기서열 분석 분리균이 분비하는 가수분해효소 분리균의 생육과 효소 생성능 결과 및 고찰 느타리버섯 수확후배지에 우점하는 CA105 균주의 분리 분리균 CA105의 동정 분리균 CA105 균주가 분비하는 cellulase와 xylanase의특성 적요 참고문헌