Earticle

초록

영어

Hesperidin is a flavanone glycoside found abundantly in citrus fruits. In vitro and in laboratory research, hesperidin has anti-inflammatory and anti-oxidant effects, reduced cholesterol and blood pressure. In this study, to investigate UVB- induced anti-oxidant and anti-aging effects of hesperidin, we measured ROS scavenging assay, matrix metalloproteinases (MMP) -1, 3, type 1 collagen (col1A1) gene expression in human dermal fibroblasts (HDFs) and NF-κB transcription activity in NIH 3T3 stable cell. Hesperidin at concentration of below 100 μM did not affect cell viability and in ROS scavenging activity, hesperidin had anti-oxidant activity in a dose-dependent manner. Futhermore hesperidin suppressed NF-κB transcription activity. To determine MMP-1, 3 and type 1 collagen gene expression is regulated by hesperidin, we performed Quantitative RTPCR (qRT-PCR) in HDFs. Levels of gene expression of MMP-1 and 3 were significantly decreased and col1A1 expression increased by hesperidin-dependently. From the above results, it is referred that hesperidin appears to have potent UVB-induced anti-oxidant and anti-anging activity. These finding suggest that hesperidin is useful ingredient for anti-aging effects of skin

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 세포배양
  2. 세포 생존율 측정
  3. 세포내 ROS 변화량 측정
  4. NF-κB luciferase assay
  5. mRNA 추출 및 cDNA 합성
  6. Quantitative real-time PCR (qRT-PCR)
 Ⅲ. 연구결과 및 고찰
  1. 세포생존율 저해 억제효과
  2. ROS 억제 효과
  3. NF-κB 전사활성 억제 효과
  4. Hesperidin에 의한 MMP1, MMP3, Col1A1 유전자 발현 변화
 Ⅳ. 결론
 참고문헌

키워드

저자

• 이현진 [ Hyunjin Lee | 건국대학교 일반대학원 생물공학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646