Earticle

초록

영어

Skin hydration is referred as the intra- and intercellular water content in the epidermis and the dermis. There are diverse in vivo assessment methods of the water content of the skin, however, in vitro assessment method of cellular water content has not been largely investigated and developed yet. Here, we developed a novel in vitro simple assessment method of cellular water content in keratinocytes using coloring silica gel. For this, we raised question about three aspects; the first is that whether the level of color change of silica gel can be correlated with the level of water content, the second is that whether the changed level of color can be quantifiable, the third is that whether the cellular water of keratinocytes can be absorbed into silica gel. We simply confirmed that its own ‘Red’ color of silica gel was changed to yellow in a H2O concentration dependent manner. Next the images of the results were adjusted to be only red and yellow color images, and then the only red color area of the images was extracted by controlling the L*a*b* values of them. Using Image J software, the intensity of the extracted red color area of each image was quantified. And we found that the level of color change is statistically correlated with a H2O concentration. Then, we investigated whether silica gel could absorb the cellular water of keratinocyte, and the color of silica gel would be changed in a keratinocyte’s water content dependent manner. The intensity level of red color area of silica gel was significantly decreased as the number of keratinocyte increased. Also the level of its red color was different between undifferentiated keratinocyte and differentiated keratinocytes. As a result, these data indicate that the cellular water of keratinocyte can be absorbed into silica gel, and the level of color change of it is statistically correlated with the level of water content of keratinocytes. Therefore, the silica gel-mediated assessment method of water content can be useful for many researches implications and for the evaluation of cosmetics.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 인간각질형성세포(keratinocyte) 배양
  2. 각질형성세포 분화(differentiation)
  3. Silica gel을 활용한 세포 수화능 측정
  4. 통계
 Ⅲ. 연구결과 및 고찰
  1. 수분량 의존적 Silica gel 변색능 확인
  2. 각질형성세포 개수 의존적 Silica gel 변색능 확인
  3. 각질형성세포 개수 의존적 Silica gel 변색능 확인
 Ⅳ. 고찰
 Ⅴ. 결론
 감사의 글
 참고문헌

키워드

저자

• 최민화 [ Min Hwa Choi | (주)한국피부임상과학연구소 ]
• 배승희 [ Seunghee Bae | 건국대학교 생물공학과 ]
• 권승빈 [ Seungbin Kwon | (주)한국피부임상과학연구소 ]
• 최영민 [ Yeong Min Choi | 건국대학교 생물공학과 ]
• 김지예 [ Ji Yea Kim | 건국대학교 생물공학과 ]
• 이준우 [ Jun Woo Lee | 건국대학교 생물공학과 ]
• 김민정 [ Min Jung Kim | 건국대학교 의학전문대학원 피부과학교실 ]
• 안규중 [ Kyu Joong Ahn | 건국대학교 의학전문대학원 피부과학교실 ]
• 안성관 [ Sungkwan An | 건국대학교 생물공학과 ]
• 안인숙 [ In-Sook An | (주)한국피부임상과학연구소 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646