Earticle

초록

영어

Present study was carried out to investigate the effect of herbal complex extract including Houttuynia cordata Thunb on hair growth and gene expression in C57BL/6 mice. Twenty mice were divided into 4 experimental groups including normal (control), 50% herbal complex extract (S-1), 100% herbal complex extract (S-2) and 5% minoxidil-treated group. The test materials were daily applied with 200 μl per mouse on shaved dorsal skin for 3 weeks. The hair growth was monitored by photograph at 7, 14 and 21 days after topical application. Then the changes of hair density and hair thickness in the hair-removed area were evaluated by phototrichogram using folliscope. Also the expression level of growth factors related to hair growth was measured by real time polymerase chain reaction. Application of S-2 or minoxidil-treated group stimulated the hair growth compared to vehicle treatment. Therefore hair density of S-2 or minoxidil-treated group application was increased about 2.5 and 3.1 fold more than in vehicle application on 21 day, respectively. And hair weight of both S-2 and minoxidil-treated group were increased about 8.3 and 10.0 fold more than in vehicle spreading on 21 day, respectively. Futhermore the gene expression of insulin-like growth factor (IGF)-1 and vascular endothelial growth factor (VEGF) were significantly up-regulated in S-2 and minoxidil-spreaded group compared to vehicle-applied group. However the gene expression of transforming growth factor (TGF)-β1 was not significantly down-regulated in S-2 and minoxidil-spreaded group compared to vehicle-applied group. These data suggest that S-2 has potent stimulating activity on hair growth in C57BL/6 mice and potential usefulness as ingredients of hair tonic and hair restore.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 시료의 준비
  2. 실험동물 및 시료의 도포
  3. 육안적 평가에 의한 발모효과의 분석
  4. 피부조직의 효소활성도 측정
  5. 실시간 정량적 중합효소 연쇄반응을 이용한 분자생물학적 분석
  6. 통계처리
 Ⅲ. 결과 및 고찰
  1. 어성초 포함 복합추출물의 육안적 발모효과 평가
  2. 털 길이 및 털 무게
  3. 피부조직의 효소활성도 변화
  4. 모발성장인자 관련 유전자 발현에 미치는 영향
 Ⅳ. 결론
 참고문헌

키워드

저자

• 홍양희 [ Yang Hee Hong | 수원여자대학교 미용예술과 ]
• 배송환 [ Song Hwan Bae | 한경대학교 식품생물공학과 ]
• 서형주 [ Hyung Joo Suh | 고려대학교 바이오시스템의과학부 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646