Xiaowan Xu, Tao Li, Ying Li, Hengming Wang, Xiaomei Xu
언어
영어(ENG)
URL
https://www.earticle.net/Article/A241829
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Pepper (Capsicum spp.) is one of the most economically and agriculturally important vegetable crops on the globe. However, the genomic resources of Pepper (Capsicum spp.) were scarcity, and only a few nucleotide sequences could be obtained in public databases. In this study, we examined transcriptome in C.annuum R597. More than 66million of high quality reads were generated using Illumina paired-end sequencing technology. Approximately 6 Gbp of data were generated, and de novo assembled into 96, 340 unigenes, with an N50 of 989 bp. The average length of unigenes was 651 bp. Based on sequence similarity search with known protein database, 51, 053 (53.0%) showed significant similarity to know proteins in Nr database, and 33, 766 (35.0%) had BLAST hits in Swiss-Prot database. Among the annotated unigenes, 26, 050 of C.annuum R597 unigenes were assigned to GO term annotation, and 28, 428 were found to have COG classification. In addition, a total of 14, 279 unigenes were assigned to 270 KEGG pathways. Moreover, a total 5, 960 SNPs, 5, 426 SSRs, and 2, 199 InDels were detected as potential molecular markers in pepper. Our study enriches the genomic resources of Pepper (Capsicum spp.) and provides powerful information for future studies. The availability of this ample amount of information about the transcriptome and SSRs, SNPs and InDels in pepper could server as valuable for studies on the agronomic traits and identification of molecular mechanism such plant resistance.
목차
Abstract Introduction Materials and Methods Plant Material and RNA Extraction CDNA Library Preparation and Sequencing De Novo Assembly and Gene Annotation of Illumina Reads Profiles of Molecular Markers Results Illumina Sequencing and de novo Assembly Annotation of Predicted Proteins Gene Ontology (GO) Annotation COG Annotation Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Mapping Molecular Markers Discovery Discussion Conclusion Acknowledgments Reference
보안공학연구지원센터(IJCA) [Science & Engineering Research Support Center, Republic of Korea(IJCA)]
설립연도
2006
분야
공학>컴퓨터학
소개
1. 보안공학에 대한 각종 조사 및 연구
2. 보안공학에 대한 응용기술 연구 및 발표
3. 보안공학에 관한 각종 학술 발표회 및 전시회 개최
4. 보안공학 기술의 상호 협조 및 정보교환
5. 보안공학에 관한 표준화 사업 및 규격의 제정
6. 보안공학에 관한 산학연 협동의 증진
7. 국제적 학술 교류 및 기술 협력
8. 보안공학에 관한 논문지 발간
9. 기타 본 회 목적 달성에 필요한 사업
간행물
간행물명
International Journal of Control and Automation
간기
월간
pISSN
2005-4297
수록기간
2008~2016
십진분류
KDC 505DDC 605
이 권호 내 다른 논문 / International Journal of Control and Automation Vol.8 No.2