Earticle

초록

영어

Nobiletin is widely used for the treatment of cancer and inflammation disorder. In neurocytes and melanocytes, nobiletin exposure induced dendrite formation. Recently, nobiletin is suggested as a new whitening agent. However, in dermis, effects of nobiletin are not understood currently. Therefore, it is important to determine how nobiletin exerts its therapeutic action and its effect on the anti-wrinkle. To determine whether MMP1 gene expression is regulated by nobiletin, we measured MMP1 mRNA expression in nobiletein-treated human dermal fibroblasts (HDFs). And we showed that transactivation activity of TPA responsive element (TRE) which is a major regulation promoter element and mitogen-activated protein kinase (MAPK) activity which main regulator of AP-1 complex. As shown results, nobiletin was decrease expression level of MMP1 mRNA in oxidative-stressed HDFs. In addition, nobiletin repressed MAPK phosphorylation and transcriptional activity of AP-1 complex in oxidative-stressed HDFs. Therefore, nobiletin protects oxidative-stress through repressing MAPK phosphorylation and transcriptional activity of AP-1 complex. In addition, these results suggest that nobiletin is a potential cosmetic ingredient repressed anti-aging and anti-wrinkle in skin.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 세포배양 및 시료
  2. MTT assay
  3. cDNA 합성 및 qRT-PCR
  4. Luciferase assay
  5. Western blotting
  6. 통계처리
 Ⅲ. 연구결과 및 고찰
  1. Nobiletin의 세포 독성 확인
  2. Nobiletin에 의한 MMP1 발현 변화
  3. Nobiletin에 의한 TPA Responsive Element (TRE)의 전사활성 변화
  4. Nobiletin에 의한 MAPKs 활성 변화
 Ⅳ. 결론
 참고문헌

키워드

저자

• 차화준 [ Hwa Jun Cha | 건국대학교 한국피부임상과학연구소 ]
• 김영주 [ Young-Joo Kim | 오산대학교 뷰티디자인계열 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646