Earticle

초록

영어

Adipose-derived stem cells (ASCs), which have characteristics of self-renewal and multipotency, are responsible for the tissue regeneration of the damaged tissue. Replicative senescence is the cause of an irreversible arrest of cell proliferation and loss cell function. Reactive oxygen species (ROS) are produced as a normal byproduct of aerobic life but the accumulation of the oxidative damage due to ROS induces the fundamental changes in cell aging process. In this study, the anti-aging effects of curcumin was studied in the long-term culture of ASCs by determining the expression of SA-β-galactosidase. Cell protection effect of curcumin was observed in UVB irradiated ASCs. Antioxidant, or anti-aging effect of curcumin by scavenging of intra-cellular ROS or enhancing the expression of antioxidant enzymes was also confirmed. These data suggest that curcumin may be effective in preventing the rise of oxidative stress during adipocyte differentiation through mechanisms that involves upregulation of endogenous antioxidant responses.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 세포주 및 세포 배양
  2. Neutral red assay
  3. ROS assay
  4. SA-β-galactosidase assay
  5. RT-PCR (Reverse transcriptase polymerase chain reaction)
  6. 통계 분석
 Ⅲ. 연구결과 및 고찰
  1. Neutral red assay
  2. ROS assay
  3. SA-β-galactosidase assay
  4. 형태학적 관찰
  5. 커큐민에 의한 항산화 효소 단백질 발현량 변화
 Ⅳ. 결론
 참고문헌

키워드

저자

• 지영은 [ Young-Eun Ji | 건국대학교 일반대학원 생물공학과 ]
• 문지선 [ Ji-Sun Moon | 건국대학교 일반대학원 생물공학과 ]
• 최태부 [ Tae-Boo Choe | 건국대학교 생물공학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646