Earticle

초록

영어

This study was carried out to investigate the antioxidative, anti-inflammatory and antimicrobial activities of Evodia officinalis extracts for cosmetic applications. The DPPH radical scavenging activity and nitrate scavenging activity of the extract (400 ug/ml) of Evodia officinalis were 91.02 and 54%, respectively. DPPH radical scavenging activity of these extracts were higher than of BHT (standard antioxidant). The inhibition effect of the extracts on ROS production was examined in H2O2-treated HaCaT cells. ROS production was significantly inhibited by the extracts of Evodia officinalis (27.5%). These inhibition effects were increased in a dose-dependent manner. Protective effect of extracts on HaCaT cells against H2O2, rotenone, and paraquat were investigated. Evodia officinalis showed protective effect against cell death of HaCaT cell induced by H2O2, rotenone, and paraquat significantly. Anti-inflammatory effect by inhibition of NO production was examined in RAW264.7 cell. Experimental results show that the NO generation is suppressed in a concentration-dependent manner These Evodia officinalis extracts were tested against Staphylococcus. epidermidis, Staphylococcus aureus, Pityrosporum ovale and along with Propionibacterium acnes, Malassezia furfur and Candida albicans which causes skin inflammation. Paper disc assay and MIC tests were carried out the various concentrations of extracts. The extracts of Evodia officinalisA displayed effective growth inhibition against S. epidermidis, P. ovale, M. furfur, with MIC value S. epidermidis (25 ㎍/㎖) P. acnes (12.5 ㎍/㎖), P. ovale (50 ㎍/㎖), M. furfur (50 ㎍/㎖). The overall results of this study indicate that the Evodia officinalis have the possibility to develop as cosmetic sources for skin anti-oxidant, skin anti-aging and treatment of skin-related diseases.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 시료조제
  2. 세포주 및 세포배양
  3. 균주 및 균주배양
  4. DPPH radical 소거작용 측정
  5. 아질산염(Nitric oxide) 소거능 분석
  6. MTT Assay를 이용한 세포독성 측정
  7. 세포내 ROS 제거능 평가
  8. 3종 chemical에 의하여 유발되는 산화적 스트레스에 대한 HaCaT 세포 보호 효과
  9. Nitrite 양의 측정
  10. 항균력 측정
  11. 최소저해농도(minimum inhibitory concentration MIC) 측정
  12. 통계분석
 Ⅲ. 연구결과
  1. 오수유에탄올 추출물의 항산화 활성
  2. 오수유에탄올 추출물의 산화적 스트레스 보호효과
  3. 오수유에탄올 추출물의 항염증 효과
  4. 피부 상재균에 대한 오수유 에탄올 추출물의 항균효과
 Ⅳ. 고찰
 Ⅴ. 결론
 참고문헌

키워드

저자

• 유민정 [ Min-Jung Ryu | 조선대학교 생명공학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646