Response Surface Methodology(RSM) was applied to enzymatic polymerization of aniline, which was conducted in a closed system containing sodium acetate buffer and presence of poly(sodium 4-styrene sulfonate)(SPS) polyelectrolyte as a template with laccase enzyme to produce water soluble polyaniline. Laccase enzyme used as catalyst was isolated from Trametes versicolor (ATCC 200801). The enzymatic polymerization rate of aniline based on measurements of initial dissolved oxygen (DO) consumption rate in the closed system, was optimized by the application of RSM. The independent variables, which had been found the most effective variables on the initial DO consumption rate by screening experiments, were determined as monomer concentration, medium temperature and medium pH. A quadratic model was developed through RSM in terms of related independent variables to describe the initial DO consumption rate as the response. Based on statistic analysis, optimum reaction conditions for the maximum initial DO consumption rate determined 42.50 mM, 39.34 °C and 5.45 as monomer concentration, medium temperature and medium pH, respectively.
목차
Abstract 1. Introduction 2. Experimental 2.1 Materials 2.2 Measurement of Laccase Activitiy 2.3 Experimental Set-up and Polyaniline Synthesis 2.4 Experimental Design and Optimization 3. Results and Discussion 3.1 Influence of Monomer Concentration and Temperature on Initial Reaction Rate 3.2 Influence of Monomer Concentration and pH on Initial Reaction Rate 3.3 Influence of Temperature and pH on Initial Reaction Rate 3.4 Selected Model Adequacy Checking 4. Conclusions Acknowledgement References
보안공학연구지원센터(IJBSBT) [Science & Engineering Research Support Center, Republic of Korea(IJBSBT)]
설립연도
2006
분야
공학>컴퓨터학
소개
1. 보안공학에 대한 각종 조사 및 연구
2. 보안공학에 대한 응용기술 연구 및 발표
3. 보안공학에 관한 각종 학술 발표회 및 전시회 개최
4. 보안공학 기술의 상호 협조 및 정보교환
5. 보안공학에 관한 표준화 사업 및 규격의 제정
6. 보안공학에 관한 산학연 협동의 증진
7. 국제적 학술 교류 및 기술 협력
8. 보안공학에 관한 논문지 발간
9. 기타 본 회 목적 달성에 필요한 사업
간행물
간행물명
International Journal of Bio-Science and Bio-Technology
간기
격월간
pISSN
2233-7849
수록기간
2009~2016
등재여부
SCOPUS
십진분류
KDC 505DDC 605
이 권호 내 다른 논문 / International Journal of Bio-Science and Bio-Technology Vol.6 No.6