Earticle

초록

영어

This study observed the effects of Ephedra sinica (E. sinica) ethanol extract in SK-MEL-2 cells on tyrosinase activity and melanin generation to identify its whitening activity and searched for changes in the protein expression of tyrosinase, TRP-1 and TRP-2 which are involved in melanin synthesis. Also, α-MSH, a melanin-stimulating enzyme was applied to skin to induce hyperpigmentation and to identify the skin whitening effect. The following results were obtained. As a result of measuring its tyrosinase inhibitory activity in vitro, it showed good inhibitory rates depending on concentration. When its effect on tyrosinase activity and melanin generation in SK-MEL-2 cell was analysed, tyrosinase activity decreased at a concentration of 50 ㎍/mL and with arbutin at 82.2%, and tyrosinase activity decreased 70.4% in E. sinica ethanol extract. As a result of measuring melanin generation, arbutin was inhibited 83.6% in 50 ㎍/mL , and 64% of E. sinica ethanol extract inhibited melanin. In investigating the effect of tyrosinase, TRP-1 and TRP-2 on protein expression in hyperpigmentation by α-MSH using western blot, E. sinica ethanol extract made the expression of tyrosinase and TRP2 decrease more significantly than arbutin. Therefore, E. sinica ethanol extract showed good effects in inhibiting tyrosinase activity and melanin generation in SK-MEL-2 cells, and inhibited melanin synthesis through inhibition of gene expression. Also, it effectively decreased the generation of tyrosinase and melanin increased by α-MSH. Based on the results above, it is suggested that E. sinica can be developed as a natural skin care material for use in whitening.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 재료 및 시료의 추출
  2. 시약
  3. 세포주 및 세포 배양
  4. 실험에 사용된 기기
  5. In vitro tyrosinase 저해활성 측정
  6. 세포 생존율 측정
  7. SK-MEL-2 세포에서 Tyrosinase 저해활성 측정
  8. SK-MEL-2 세포에서 Melanin 생합성 저해율 측정
  9. α-MSH에 의한 Tyrosinase 활성 및 과생성 Melanin에 미치는 영향 분석
  10. Western blot 분석
  11. 통계분석
 Ⅲ. 연구결과 및 고찰
  1. In vitro tyrosinase 저해 활성
  2. 추출물에 의한 SK-MEL-2 세포생존율
  3. 추출물이 SK-MEL-2 세포내의 Tyrosinase 활성도 및 Melanin 생성량에 미치는 영향
  4. 추출물이 Melanin 합성 관련 단백질 발현에 미치는 영향
  5. α-MSH 유도에 의한 Tyrosinase 활성도 및 Melanin 생성량에 미치는 추출물의 효과
  6. α-MSH의 과색소침착 시 Melanin 합성 관련 단백질 발현에 미치는 추출물의 영향
 Ⅳ. 결론
 참고문헌

키워드

저자

• 정명아 [ Myung-A Jeong | 청암대학교 향장피부미용과 ]
• 오수정 [ Su-Jeong Oh | 조선이공대학교 뷰티아트과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646