Earticle

초록

영어

Although previous studies have identified that both the fruit and leaf of dangyuja (Citrus grandis Osbeck) exhibits anticancer, antioxdant and anti-inflammatory effect, the inhibitory effect of melanogenesis have not been reported. In this study, freeze dried extract of dangyuja was evaluated for their potential to inhibit melanogenesis. Results show that, freeze dried extracts of dangyuja strongly decreased the tyrosinase activity for catalysis of L-dihydroxyphenylalanine (L-DOPA) oxidation in a dose–dependent manner. In addition, we also verified that cytotoxicity and melanin content in mouse B16F10 melanoma cells were assayed after treatment with the freeze dried extracts of dangyuja. The results demonstrate that extracts possessed low cytotoxic activity and that melanin content was reduced. Overall, freeze dried extracts of dangyuja is a safe natural materials that show an effective inhibition of melanogenesis. These results suggest that freeze dried extracts of dangyuja can be considered as an effective skin whitening reagent for application in cosmetics.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 당유자 미숙과 동결건조 추출물의 제조
  2. 당유자 미숙과 동결건조 추출물 분석
  3. In vitro DOPA 산화활성 저해시험
  4. 세포 배양
  5. 세포 독성 시험
  6. 세포 내의 멜라닌 생성 저해시험
 Ⅲ 연구결과 및 고찰
  1. 당유자 미숙과 동결건조 추출물의 함량 분석
  2. In vitro DOPA 산화활성 저해시험
  3. 시험물질의 검액농도 결정
  4. 세포 내의 멜라닌생성 저해시험
 Ⅳ. 결론
 참고문헌

키워드

저자

• 김가람 [ Karam Kim | 건국대학교 한국피부임상과학연구소 ]
• 차훈 [ Hun Cha | (주)더마펌 ]
• 송희경 [ Hee-Kyung Song | 건국대학교 산업대학원 향장학과 ]
• 이유나 [ Yu Na Lee | 건국대학교 의학전문대학원 피부과학교실 ]
• 안규중 [ Kyu Joong Ahn | 건국대학교 의학전문대학원 피부과학교실 ]
• 안인숙 [ In-Sook An | 건국대학교 한국피부임상과학연구소, 건국대학교 산업대학원 향장학과 ]
• 안성관 [ Sungkwan An | 건국대학교 한국피부임상과학연구소, 건국대학교 산업대학원 향장학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646