Earticle

초록

영어

Attention deficit/hyperactivity disorder (ADHD) is a neurobehavioral disorder of cognition. We investigated the effects of treadmill exercise on Purkinje cell and astrocytic reaction in the cerebellum of the ADHD rat. Adult male spontaneously hypertensive rats (SHR) and Wistar-Kyo-to rats (WKYR) weighing 210±10 g were used. The animals were ran-domly divided into four groups (n=15): control group, ADHD group, ADHD and methylphenidate (MPH)-treated group, ADHD and treadmill exercise group. The rats in the MPH-treated group as a positive control received 1 mg/kg MPH orally once a day for 28 consecutive days. The rats in the treadmill exercise group were made to run on a treadmill for 30 min once a day for 28 days. Motor coordination and balance were determined by vertical pole test. Immunohistochemistry for the expres-sion of calbindinD-28 and glial fibrillary acidic protein (GFAP) in the cer-ebellar vermis and Western blot for GFAP, Bax, and Bcl-2 were con-ducted. In the present results, ADHD significantly decreased balance and the number of calbindin-positive cells, while GFAP expression and Bax/Bcl-2 ratio in the cerebellum were significantly increased in the ADHD group compared to the control group (P<0.05, respectively). In contrast, treadmill exercise and MPH alleviated the ADHD-induced the decrease of balance and the number of calbindine-positive cells, and the increase of GFAP expression and Bax/Bcl-2 ratio in the cerebellum (P<0.05, respectively). Therefore, the present results suggested that treadmill exercise might exert ameliorating effect on ADHD through re-duction of Purkinje cell loss and astrocytic reaction in the cerebellum.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Experimental animals and treatments
  Vertical pole test
  Treadmill exercise protocol
  Tissue preparation
  Immunohistochemistry for calbindinD-28k and GFAP
  Western blot analysis
  Data analysis
 RESULTS
  Effect of treadmill exercise on balance in the vertical pole test
  Effect of treadmill exercise on survival of Purkinje cells in the cerebellum
  Effect of treadmill exercise on GFAP protein expression in the cerebellum
  Effect of treadmill exercise on Bax and Bcl-2 protein expression in the cerebellums
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Hyo-Soon Yun [ Dague Veterans Hospital, Daegu, Korea ]
• Mi-Sook Park [ Department of Health and Welfare for the Elderly, Graduate School, Daegu Haany University, Gyeongsan, Korea ]
• Eun-Sang Ji [ Department of Sport & Health Science, College of Natural Science, Sangmyung University, Seoul, Korea ]
• Tae-Woon Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Il-Gyu Ko [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Hyun-Bae Kim [ Department of Taekwondo, College of Physical Education, Kyung Hee University, Seoul, Korea ]
• Hong Kim [ Department of Oriental Sports Medicine, College of Biomedical Science, Daegu Haany University, Gyeongsan, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613