Earticle

초록

영어

Adipogenesis was defined as a differentiation process of preadipocytes into adipocytes. It is a key process in determining the number of mature adipocytes in the development of obesity. Thus, to regulation of adipogenesis is crucial target in obesity prevention. Mussel (Mytilus corscus ) has been reported to possess abundant dietary proteins and savory amino acids. However, antiadipogenic role and function in obesity prevention remains unknown. Here, we employed green-shelled mussel and its water extract (GME) was prepared. To elucidate the potential role of GME in 3T3-L1 adipogenesis, 3T3-L1 cells were treated with GME between day –2 to day 6. We found that GME dose-dependently suppressed adipogenesis and inhibited formation of lipid droplets accompanied by reduced level of adipogenic transcriptional factors such as C/EBPα and FAS. Our study further revealed that inhibitory function of GME wa largely limited to the adipogenesis day 0 to day 6. Taken together, our evidences provide new insights into the molecular basis underlying the antiadipogenic function of GME.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 실험 재료
  2. 녹색입 홍합 추출물의 제조
  3. 3T3-L1 지방세포분화
  4. 3T3-L1 지방세포 분화모델에서 GME의 항비만 활성평가
  5. 3T3-L1 세포내 지방구 관찰
  6. PCR을 통한 adipogenic 유전자의 발현수준 분석
  7. 통계분석
 Ⅲ. 연구결과 및 고찰
  1. GME의 지방세포 분화억제 효능평가
  2. Microscopy observation
  3. GME가 3T3-L1 adipogenic 유전자 발현에 미치는 영향
  4. 처리 시기에 따라 GME가 adipogenesis에 미치는 영향
 Ⅳ. 결론
 참고문헌

키워드

저자

• 손명수 [ Myung-Soo Shon | 경남대학교 식품생명학과 ]
• 김시경 [ Si-Kyung Kim | 경남대학교 식품생명학과 ]
• 이승철 [ Seung-Cheol Lee | 경남대학교 식품생명학과 ]
• 김교남 [ Gyo-Nam Kim | 경남대학교 식품생명학과 ] Corresponding authors

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646