In order to study about human skin anti-wrinkle effect of red ginseng, red ginseng extracts and the fractions were tested to inhibition effect against MMP-2, -9, and -1 expression in human fibroblast cells by gelatin zymography and RT-PCR methods. The red ginseng samples are red ginseng extract extracted with hot water, crude saponin as butanol soluble fraction, major ginsenosides, protopanaxatrol conversions, protopanaxadiol conversions as the fractions fractionated by C18 revers phase resin and various proportions of aqueous acetonitrile. We found that propanaxatriol conversions fraction one of the red ginseng samples inhibited in a concentration-dependent manner against MMP-2, and -9 expression levels in human fibroblast cells irradiated by UV light. By HPLC analysis, protopanaxtriol conversions fraction consisted on ginsenosides Rk3, F4, Rg6, and Rh4. These results suggest that dehydrates of protopanaxatriol ginsenoside from red ginseng have an antiwrinkle activity through inhibition of MMP-2, and -9 expression in skin.
목차
Abstract Ⅰ. 서론 Ⅱ. 연구재료 및 방법 1. 시료제조 2. 세포독성 3. 역전사 중합반응(RT-PCR)에 의한 MMP-1 발현억제 실험 4. Gelatin zymography를 이용한 MMP-2, 9 효소 활성시험 Ⅲ. 연구결과 및 고찰 1. 활성 사포닌분획의 진세노사이드 조성 2. 세포독성 3. 홍삼 각 시료에 대한 MMP-1 발현억제능 4. 홍삼 각 시료에 대한 MMP-2, 9 효소 활성 Ⅳ. 결론 참고문헌
키워드
Panax ginsengProtopanaxatriolMMPsAnti-wrinkle
저자
이상명 [ Sang Myung Lee | 한국인삼공사 중앙연구소 ]
임영호 [ Young Ho Im | 한국인삼공사 중앙연구소 ]
김석창 [ Seok Chang Kim | 한국인삼공사 중앙연구소 ]
최재환 [ Jae Hwan Choi | 한국인삼공사 중앙연구소 ]
김진희 [ Jin Hee Kim | 대구한의대학교 한방피부미용학과 ]
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