Earticle

초록

영어

The effect of curcumin, a main component of Curcuma longa , on the MMP-1 and collagen production in human dermal fibroblast was studied (to observe the availabilty for) as a raw material of cosmetics. The effect of curmumin has found that expression of MMP-1 was reduced at lower concentration of curcumin by using HDF cells that UVB (100 mJ/cm²) was investigated. Enhanced collagen production was confirmed by repeated tests. Because curcumin affect JNK and p38 and plays the role of hindering PI3K as collagen increases at curcumin concentration of 10 to 11 μM and revelation of collagen is increased to in the group. Actually, the wstern blot result has found that phosphorylation of JNK was increased when 10 μM of curcumin concentration was treated. AKT has found that phosphorylation of AKT decreased as the downstream gene of PI3K. It is thought that these results are the same one that collagen production is increased as curcumin affect JNK and curcumin plays the role of PI3K inhibitors depending on concentration. On the other hand, the decrease of collagen production at the higher concentration is not clear yet and further studies are necessary (to elucidate the reason).

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 실험 재료 및 세포배양
  2. Neutral Red assay를 이용한 세포 독성 검사
  3. ELISA (Enzyme-linked immunosorbent assay)
  4. Western blot
  5. RT-PCR (Reverse transcriptase polymerase chain reaction)
 Ⅲ. 연구결과 및 고찰
  1. 커큐민이 HDF 세포에서 MMP-1 발현에 미치는 영향
  2. 커큐민이 HDF 세포에서 콜라겐 생성에 미치는 영향
 Ⅳ. 결론
 참고문헌

키워드

저자

• 정은진 [ Eun-Jin Jung | 건국대학교 일반대학원 생물공학과 ]
• 문지선 [ Ji-Seon Moon | 건국대학교 일반대학원 생물공학과 ]
• 최태부 [ Tae-Boo Choe | 건국대학교 생물공학과 ] Corrrsponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646