Earticle

초록

영어

This study was evaluated antioxidant activity of ethanol extracts of Juglans regia (walnut) husk (WHE) using 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. Also, cell viability of WHE on human keratinocyte (HaCaT) was evaluated by sulforhodamine B (SRB) assay. Inhibitory effect of WHE on tumor necrosis factor alpha (TNF-α) induced Matrix Metalloproteinase-1 (MMP-1 ) expression in HaCaT was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). As the results, WHE was showed similar antioxidant activity to vitamin C at concentration of 6.25, 12.5, 25, 50 μg/mL. WHE of low concentration (6.25, 12.5 and 25 μg/mL) didn’t exhibited cytotoxicity in HaCaT. However, treatment of 50 and 100 μg/mL WHE were showed cell viability of about 98.4% and 91.1%, respectively, in same cells. Matrix Metalloproteinase-1 (MMP-1 ) mRNA were induced by TNF-α in HaCaT cells and WHE treatment of 10, 20, 50 and 100 μg/mL were decreased MMP-1 mRNA with dose-dependent manner. Exhibiting excellent antioxidant activity with high cell viability, and potent inhibitory effect on MMP-1 mRNA, ethanol extracts of walnut husk extracts can be applied in cosmetics as a MMP -1 inhibitor.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 시약
  2. 호두 껍데기 추출물 제조
  3. 세포배양
  4. 향산화 효과
  5. 세포 생존율 측정
  6. MMP-1 저해활성 측정
 Ⅲ. 연구결과 및 고찰
  1. 항산화 효과
  2. 세포 생존율 측정
  3. MMP-1 저해 효과
 Ⅳ. 결론
 참고문헌

키워드

저자

• 박장순 [ Jang-Soon Park | 동원대학교 뷰티디자인계열 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646