Earticle

초록

영어

In this study, we investigated effects of the complex material containing Centella asiatica (C. asiatica )- and folic acidfermented extracts, acetyl glutamine, and nicotinic acid adenine dinucleotide phosphate (NAADP) on the improvement of skin condition and skin cell functions. We confirmed cellular effects of fermented extracts of C. asiatica and folic acid using microorganism mixture (CFFE) and acetyl glutamine- and NAADP-containing CFFE (FAN) on cell viability, reactive oxygen species (ROS) generation, cellular senescence, DNA repair, transcriptional activity of NF-κB, regulation of gene expression, cellular regeneration and migration, cell differentiation, and melanin contents in human dermal fibroblasts (HDFs), HaCaT human keratinocytes, B16F10 mouse melanocytes, and NIH-3T3 mouse embryo fibroblasts. CFFE and FAN showed significant protection effects against cellular damage caused by ultraviolet B (UVB) irradiation or ROS generation. The expression levels of Col1A1 was up-regulated and of MMP1 was down-regulated by treatment of CFFE and FAN in UVB-exposed HDFs. UVB-induced NF-κB activation was significantly suppressed by treatment of CFFE and FAN in NIH-3T3 cells. Also the treatment of CFFE and FAN promoted the differentiation on HaCaT cells. The CFFE and FAN suppressed production of melanin in B16F10 cells by inhibition of expression of MITF and TYR. Especially, it was observed that acetyl glutamine and NAADP increase overall effects of CFFE in earlier time points, implicating that these components initiate and boost cellular signals and positive functions mediated by CFFE in the skin. Taken together, these findings suggest that CFFE and FAN might have excellent efects of the anti-aging, reduction of wrinkle and pigmentation , promotion of cellular regeneration, improvement of epidermal barrier quality, and anti-inflammation on the skin cell.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 원료 제조
  2. 피부 세포 배양
  3. 세포 생존율 측정
  4. ROS 변화량 측정
  5. DNA 복구 능력 측정
  6. 세포노화 측정
  7. qRT-PCR을 통한 유전자 발현 확인
  8. NF-κB luciferase assay를 통한 NF-κB 전사 활성 변화 측정
  9. 세포재생 및 세포이동 능력 측정
  10. Western blot을 활용한 단백질 발현 변화 측정
  11. 멜라닌 생성량 측정
 Ⅲ. 연구결과
  1. 세포 생존률에 미치는 영향
  2. 세포노화에 미치는 영향
  3. 진피섬유아세포의 생리활성에 미치는 영향
  4. 염증반응 억제 효과
  5. 각질형성세포의 생리활성에 미치는 영향
  6. 멜라닌형성세포의 생리활성에 미치는 영향
 Ⅳ. 고찰
 Ⅴ. 결론
 참고문헌

키워드

저자

• 윤영민 [ Yeongmin Yoon | 건국대학교 생물공학과 ]
• 윤정연 [ Jeung Yeun Yoon | 건국대학교 생물공학과 ]
• 임경미 [ Kyung Mi Lim | 건국대학교 생물공학과 ]
• 한형진 [ Hyung Jin Hahn | 건국대학교 의학전문대학원 피부과학교실 ]
• 김유리 [ Yu Ri Kim | 건국대학교 의학전문대학원 피부과학교실 ]
• 안규중 [ Kyu Joong Ahn | 건국대학교 의학전문대학원 피부과학교실 ]
• 안성관 [ Sungkwan An | 건국대학교 생물공학과, 건국대학교 산업대학원 향장학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646