Earticle

초록

영어

Nanoencapsulation is the coating of various substances within another material at sizes on the nanoscales and it is prospected in all kinds of industries especially cosmetics, pharmaceutics, and food manufacturing. This study was performed to investigate the antioxidant activities as comparing ethanol extracts (ME) and nano-encapsulation of Mulberry Root Bark Extracts by lecithin and cholesterol (NM). DPPH radical scavenging activity of both extracts are almost identical for the 250 ug/ml samples. Also, the effects on nano- encapsulated extracts (NM) and ethanol extracts (ME) on the generation (induction) of Reactive Oxygen Species (ROS) by adding LPS to induce oxygen stress into the cell was that the both extracts in adding sample (500 ㎍/ ㎖) or (1000 ㎍/㎖) showed similar to oxidative stresses inhibition compared to 50% of control. ln addition, cell viability of the nano-encapsulated extracts (NM) was measured on RAW 264.7 cells and showed higher than that of ethanol extract (ME) in each concentration. The size of the nano-encapsulated extracts (NM) was an average diameter of range of 200-400 nm so they can effectively penetrate cell more than ethanol extract (ME) as nano-encapsulated extracts (NM) have great mobility and affinity for cell because of their small size. it was observed by confocal laser scanning microscope.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 시료 및 추출
  2. 나노입자 제조 및 방출
  3. 세포배양
  4. 활성산소 생성 저해 활성 - DCFH - DA assay
  5. 항산화 효과 - DPPH radical 소거 활성
  6. 세포생존율 측정 - MTT assay
  7. 세포 투과 관찰 - Confocal laser 주사현미경
 Ⅲ. 연구결과 및 고찰
  1. 나노입자의 확인
  2. 활성산소 생성 저해 활성 - DCFH - DA assay
  3. 항산화 효과 - DPPH radical 소거 활성
  4. 세포생존율 측정 - MTT assay
  5. 세포 투과 관찰 - Confocal laser 주사 현미경
 Ⅳ. 결론
 참고문헌

키워드

저자

• 권혁년 [ Hyuk-Nyun Kwon | 전북대학교 수의과대학 공중보건학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646