Earticle

초록

영어

The purpose of this study was to investigate whitening agent from a natural substance using in vivo model zebrafish. Tyrosinase is a key enzyme in melanogensis which was used for screening of whitening agent with tyrosinase inhibition assay and melanocyte cell melanin biosynthesis inhibition assay. However, pigmentation mechanisms were very a complicated procedure in human dermis. In this study, we used zebrafish embryo as an animal model for confirmed phenotype-based screening of melanin synthesis with natural sources. Zebrafish has most of same organs and tissues that can be seen in human. Especially, pigments were located in the epidermal layer on the surface of organism. In order to examine the whitening effects involved in pigment cell signaling, we treated zebrafish embryos with 10 kinds of methanol extracts of medicinal herbs. Among those medicinal herbs tested, 50 μg/mL of MeOH extract of Forsythia suspensa was decreased the pigmentation on zebrafish larva. After solvent partitioning with MeOH extract of F. suspense , chloroform and ethyl acetate fractions showed the depigmentation effect on zebrafish. In contrast the tyrosinase inhibition effect of F. suspense has not shown. Those results suggest that different approaches to obtain depigmentation process, and confirm the new protocols to evaluate mleanogenesis using in vivo zebrafish model.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 재료 및 시료 추출
  2. Tyrosinase 활성 억제 실험
  3. Zebrafish 사육
  4. Zebrafish melanin 저해 실험
  5. 통계처리
 Ⅲ. 연구결과 및 고찰
  1. 생약재의 tyrosinase 억제 활성
  2. Zebrafish melanin 생합성 억제 활성
 Ⅳ. 결론
 감사의 글
 참고문헌

키워드

저자

• 백승화 [ Seung Hwa Baek | 경북대학교 식품공학부 ]
• 박종호 [ Jong Ho Park | ㈜다인피아 ]
• 김진희 [ Jin Hee Kim | 대구한의대학교 한방피부미용학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646