Earticle

초록

영어

Adipogenesis was defined as a differentiation process of preadipocytes into adipocytes and its regulation is crucial in obesity prevention. Several transcriptional factors such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein family (C/EBPs) were known as a key factors in early stage of adipogenesis. Selenium, an essentional micronutrient, has been reported to possess nurmerous biological activities. However, anti-obese function of selenium, particularly selenate, is relatively unknown. In this study, we investigated that inhibitory activities of selenate against adipocytes differentiation of C3H10T1/2 cells. Selenate treatment showed no cytotoxicity up to 100 μM in C3H10T1/2 cells. It showed dose-dependent suppression of adipogenesis between 1 to 50 μM. We also found that dose-dependently inhibited adipogenesis of C3H10T1/2 cells accompanied by reduced expression levels of PPARγ and C/ EBPβ as judged by PCR analysis. Our results imply that the inhibitory function of selenate is associated with the suppression of adipogenic genes in early stage of adipogenesis. These scientific evidences suggest that selenate could be used in development of obesity and/or overweight-related nutrient supplement and nutricosmetics.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 실험 재료
  2. C3H10T1/2의 지방세포분화
  3. C3H10T1/2 세포에서 selenate의 세포독성
  4. Oil Red O (ORO) 염색법에 의한 항비만 활성평가
  5. PCR을 통한 전사인자의 발현수준 분석
  6. 통계분석
 Ⅲ. 연구결과 및 고찰
  1. C3H10T1/2 세포에서 selenate의 세포독성
  2. Selenate의 지방세포 분화 억제 효능
  3. Selenate 처리에 의한 C3H10T1/2 세포의 전사인자 발현
 Ⅳ. 결론
 감사의 글
 참고문헌

키워드

저자

• 손명수 [ Myung-Soo Shon | 경남대학교 식품생명학과 ]
• 송지혜 [ Ji-Hye Song | 한남대학교 식품영양학과 ]
• 김교남 [ Gyo-Nam Kim | 경남대학교 식품생명학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646