Earticle

초록

영어

Diabetic retinopathy is one of the most important microvascular compli-cations in diabetes, and it is the major cause of visual loss. Physical ex-ercise is known to ameliorate the symptoms of metabolic syndromes such as diabetic mellitus. In the present study, we investigated the ef-fects of treadmill exercise on vascular endothelial growth factor (VEGF) expression and apoptotic cell death in the retinas of streptozotocin (STZ)- induced diabetic rats. The male Sprague- Dawley rats were randomly divided into three groups (n=10 in each group): control group, STZ-in-duce diabetes group, STZ- induced diabetes and treadmill exercise group. To induce diabetes in the experimental animals, a single intra-peritioneal injection of STZ (50 mg/kg) was given to each animal. The rats in the exercise group were forced to run on a motorized treadmill for 30 min once a day during 1 week starting 6 weeks after STZ injec-tion. In the present results, VEGF expression in the retinas was increased by induction of diabetes. The numbers of caspase-3-positive and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)- positive cells in the retinas were also enhanced by induction of diabe-tes. Treadmill exercise significantly decreased VEGF expression and suppressed the number of TUNEL-positive and caspase-3-positive cells in the retinas of diabetic rats. In the present study, we have shown that treadmill exercise might alleviate the progression of diabetic retinopa-thy through suppressing VEGF expression and apoptotic cell death in the retinas of the diabetic rats.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Animals and treatments
  Induction of diabetes
  Treadmill exercise
  Tissue preparation
  Caspase-3 immunohistochemistry
  TUNEL staining
  Western blot for VEGF expression
  Data analysis
 RESULTS
  Effects of treadmill exercise on body weight and blood glucose level
  Effects of treadmill exercise on VEGF expression in the retinas
  Effects of treadmill exercise on DNA fragmentation in the retinas
  Effects of treadmill exercise on caspase-3 expression in the retinas
 DISCUSSION
 CONFLICT OF INTEREST
 REFERENCES

키워드

저자

• Eun-Sang Ji [ Department of Sport & Health Science, College of Natural Science, Sangmyung University, Seoul, Korea ]
• Il-Gyu Ko [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Jung-Wan Cho [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Ronald W. Davis [ Department of Kinesiology, College of Health Sciences, Texas Woman’s University, Texas, USA ]
• Gwang-Yon Hwang [ Department of Kinesiology, College of Health Sciences, Texas Woman’s University, Texas, USA ]
• Yong-Seok Jee [ Department of Leisure Sports, College of Science, Hanseo University, Seosan, Korea ]
• Baek-Vin Lim [ Department of Exercise Prescription, Division of Leisure & Sports Science, Dongseo University, Busan, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613