Earticle

초록

영어

To evaluate the cytotoxicity and protective effect of Smilax china L. (SC) extract on ferrous sulfate (FeSO4), an autooxidant of melanin formation, cell viability were analysed by XTT assay after human skin melanoma cells (SK-MEL-3) were cultured in media containing various concentrations of FeSO4. And also, the effect of antioxidant α-tocopherol on FeSO4-induced cytotoxicity was assessed. For the protective effect of SC extract on FeSO4-induced cytotoxicity, SK-MEL-3 cells were pretreated with 50 or 80 μg/㎖ of SC extract for 2 h before the treatment of FeSO4. And also, the antioxidative effects of SC extract were assessed by latate dehydrogenase (LDH) activity. In this study, FeSO4 remarkably decreased cell viability dose-dependent manner compared with control. And the XTT50 value was determined at 62.0 μM of FeSO4. In the effect of antioxidant, α-tocopherol effectively prevented FeSO4-induced cytotoxicity by the significant increase of cell viability. In the protective effect of SC extract on FeSO4-induced cytotoxicity, SC extract remarkably increased cell viability which was decreased by FeSO4-induced cytotoxicity, and also it showed the antioxidative effects such as a significant decrease of LDH activity. In the melanin generation, SC extract effectively blocked melanin generation by the decrease of tyrosinase activity and total amount of melanin. From these results, it is suggested that the cytotoxicity of FeSO4 was involved in oxidative stress, and also, SC extract effectively prevented the cytotoxicity and melanogenesis induced by an autooxidant of melanin formation, FeSO4 through antioxidative effect. Conclusively, SC extract may be a putative resources as an protective agent for oxidative stress-mediated skin hyperpigmentation via hyperactivity of autooxidant in melanin formation.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 약제제조
  2. 세포배양
  3. 청미래덩굴(SC) 추출
  4. 자동산화제의 처리
  5. 항산화제의 처리
  6. SC 추출물의 처리
  7. 세포생존율(cell viability) 측정
  8. Lactate dehydrogenase (LDH) 활성 측정
  9. 티로시나제 활성
  10. 총멜라닌양 측정
  11. 통계처리
 Ⅲ. 결과 및 고찰
  1. FeSO4의 세포생존율 측정
  2. FeSO4에 대한 항산화제의 영향
  3. SC 추출물이 자동산화제에 미치는 영향
  4. LDH 활성 측정
  5. 티로시나제(tyrosinase)활성 측정
  6. 총멜라닌양 측정
 Ⅳ. 결론
 감사의 글
 참고문헌

키워드

저자

• 표애자 [ Ae-Ja Pyo | 원광대학교 대학원 자연치료요법학과 ]
• 윤미영 [ Mi-Young Yoon | 원광대학교 대학원 자연치료요법학과 ]
• 양현옥 [ Hyun-Ok Yang | 원광보건대학교 미용피부관리학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646